An increase in the number of outbreaks of foodborne disease associated with fresh produce consumption has been described. The objective of the present study was to evaluate the effect of water suspended particles during immersing/spraying disinfection processes and the recovery of Cryptosporidium parvum oocysts from tomato surfaces. Tomatoes (Lycopersicum esculetum Mill.) were immersed/sprayed ...

Cross-transmission experiments were performed using oocysts of an Eimeria arizonensis-like coccidian from Peromyscus leucopus and Peromyscus truei, an E. arizonensis-like coccidian from Reithrodontomys fulvescens, Eimeria baiomysis and Eimeria taylori from Baiomys taylori, Eimeria albigulae from Neotoma albigula, and Eimeria onychomysis from Onychomys spp., between representatives of the above ...

Sanda A., Salman Al Said: Intratracheal Infection of Chickens with Sporulated Oocysts of Eimeria tenella. Acta vet. Bmo, 47, 1978: 185-187. Experiment involving 16 treated and 10 control chickens, aged 3 weeks, was conducted to study the intratracheal route of infection by sporulated oocysts of Eimeria tenella. In the experimental birds a surgical procedure was conducted to prevent ingestion of...

A computer-assisted laser scanning microscope equipped for confocal laser scanning and color video microscopy was used to examine Cryptosporidium parvum oocysts in two agricultural soils, a barnyard sediment, and calf fecal samples. An agar smear technique was developed for enumerating oocysts in soil and barnyard sediment samples. Enhanced counting efficiency and sensitivity (detection limit, ...

OBJECTIVE To estimate the analytic sensitivity of microscopic detection of Toxoplasma gondii oocysts and the environmental loading of T gondii oocysts on the basis of prevalence of shedding by owned and unowned cats. DESIGN Cross-sectional survey. SAMPLE POPULATION 326 fecal samples from cats. PROCEDURES Fecal samples were collected from cat shelters, veterinary clinics, cat-owning househ...

The use of nucleic acid staining with a fluorochrome dye to differentiate viable and dead (heat-killed) Cryptosporidium oocysts was assessed. The specificities (percentage of unstained viable oocysts) and sensitivities (percentage of stained dead oocysts) of the seven tested dyes (SYTO-17® and SYTO-59® to 64®) ranged from 65 to 76% (average 71%) and 83 to 95% (average 91%), respectively. SYTO-5...

Cryptosporidium is a genus of waterborne protozoan parasites that causes significant gastrointestinal disease in humans. These parasites can accumulate in environmental biofilms and be subsequently released to contaminate water supplies. Natural microbial assemblages were collected each season from an eastern Pennsylvania stream and used to grow biofilms in laboratory microcosms in which influx...

Swine toxoplasmosis is one of the most important diseases of pigs in Japan. Toxoplasma is a causal agent of zoonoses infecting not only swine, but also many kinds of mammals, including man. Therefore, routes of infection have been examined in toxoplasmosis of man and animals. Congenital transmission has already been confirmed in man and animals, but no acquired infection has been fully understo...

In the intestine of 10 (n = 18) cyprinid fish species collected from natural waters of Hungary, the author demonstrated oocysts which were shed unsporulated In tapwater, oocysts sporulated within 24 to 48 h In 6 of the fish species, oocysts of 2 sizes were observed Histological examnations proved that, of the morphologically similar oocysts larger ones developed in nodules in size of a pinhead ...

The survival of Cryptosporidium parvum oocysts in soil and water microhabitats may be affected by the environmental production and release of free ammonia. The objective of this study was to determine the effects of increasing free ammonia concentrations and times of exposure on oocyst viability. Wild-type oocysts were obtained from naturally infected calf feces by chemical (continuous-flow) ce...