In Benin, schools become sometimes the hub for dealing and consuming psychoactive substances; opioids in particular. The objectives of this study are to identify risk factors related use investigate genetic polymorphism mu delta opioid receptors teenagers young adults who consume schools. To accomplish this, 453 students participated study; R diversity 3.6.1 software RStudio environment was use...

The activity of imipenem against Pseudomonas aeruginosa HUS-3 decreased by 16 times in the presence of substances eluted from siliconized latex urinary catheters (SLUCs). SLUCs did not inactivate imipenem or increase beta-lactamase activity. The outer membrane of P. aeruginosa HUS-3 grown in the presence of eluate lacked an OprD-like protein and expressed a new 50-kDa protein. The decreased act...

Glyoxysomes of endosperm tissue of castor bean (Ricinus communis L.) seedlings were solubilized in a detergent and added to a lipid bilayer. Conductivity measurements revealed that the glyoxysomal preparation contained a porin-like channel. Using an electrophysiological method, which we established for semiquantitative determination of porin activity, we were able to demonstrate that glyoxysoma...

susceptibility test were performed on Mueller-Hinton agar with 5% horse blood according to NCCLS recommendations with the following discs: cefuroxime (30/jg), ceftizoxime (30 fig), cefpodoxime (30 /ig), cefixime (30 fig), cefaclor (30 ug) and loracarbef (10 and 30/ig). Agar and discs were from Sanofi Diagnostic Pasteur (Marnes-la-Coquette, France) except loracarbef prepared from standard refere...

Carbapenem antibiotics have been shown to penetrate the outer membrane of Pseudomonas aeruginosa through a unique porin protein, OprD2. We mapped the OprD2 gene by conjugation using plasmid FP2 and by transduction using phage F116L. This gene maps between 71 and 75 min on the PAO1 chromosome.

Transposon-directed cloning was used to isolate the ompL gene from the deep-sea bacterium Photobacterium species strain SS9. The deduced amino acid sequence of OmpL displays sequence homology to porin proteins from enteric bacteria. Gene fusion and primer extension analyses indicate that ompL is transcriptionally regulated by pressure.

Porin, also termed the voltage-dependent anion channel, is the most abundant protein of the mitochondrial outer membrane. The process of import and assembly of the protein is known to be dependent on the surface receptor Tom20, but the requirement for other mitochondrial proteins remains controversial. We have used mitochondria from Neurospora crassa and Saccharomyces cerevisiae to analyze the ...

Voltage-dependent anion channel (VDAC) has been suggested to be a mediator of mitochondrial-dependent cell death induced by Ca(2+) overload, oxidative stress and Bax-Bid activation. To confirm this hypothesis in vivo, we generated and characterized Drosophila VDAC (porin) mutants and found that Porin is not required for mitochondrial apoptosis, which is consistent with the previous mouse studie...