objective: this study was designed to evaluate the effects of vitrification and in vitro culture of human ovarian tissue on the expression of oocytic and follicular cell-related genes. materials and methods: in this comparative study, ovarian tissue samples were obtained from eight transsexual women. samples were cut into small fragments and were then assigned to vitrified and non-vitrified gro...

OBJECTIVE To evaluate the integrity of genomic imprinting in oocytes vitrified at the germinal vesicle (GV) stage and in vitro matured (IVM) after thawing. DESIGN Clinical research and application. SETTING University-based fertility center. PATIENT(S) Immature oocytes were donated for research by patients who were included in an intracytoplasmic sperm injection program. INTERVENTION(S) ...

Background: 15% of oocytes collected in ART cycles are immature. These oocytes may be matured following IVM program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM. The aim was to determine the maturation rate, morphology and viability of human immature oocytes after fresh IVM and vitrified-IVM program. Materials and Methods: 63 women who und...

Background: Since the freezing method was offered, much effort has been done to improve the freezing conditions in different studies and the impact of different freezing methods and mediums on oocyte, embryo and ovarian tissues has been examined. Based on studies, minerals play an important role in oocyte maturation and subsequent embryo development. Vanadium is one of the minerals, which is ve...

Abstract Study question Which are the potential effects of vitrification on ovarian tissue, isolated follicles and short-term cultured follicles? Summary answer Vitrification-warming protocol using ethylene glycol as a cryoprotectant causes no severe damage to tissue or follicles. What is known already Ovarian cryopreservation (OTC) used for fertility preservation (the only possible approach ap...

OBJECTIVE To evaluate a cryopreservation technique by vitrification of whole ovaries with their vascular pedicle in sheep, by using two cryoprotectant solutions. DESIGN Animal study. SETTING Fertility clinic in a university teaching hospital. ANIMAL(S) Five to 6-month-old ewes. INTERVENTION(S) Whole sheep ovaries with their vascular pedicles were collected at the slaughterhouse and prep...

background: one of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. matrix metalloproteinases 2 (mmp-2) and 9 (mmp-9) and their tissue inhibitors timp-1 and timp-2 are involved in the remodeling of the extracellular matrix in the ovaries. objective: this study aimed to evaluate the expression of mmp-2, mmp-9, timp-1, and timp-2 genes ...

Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...

objective: this study aimed to evaluate the incidence of apoptosis in vitrified and non-vitrified human ovarian tissue by the use of morphological analysis and apoptosis assay techniques. methods: we obtained human ovarian tissue biopsies from 30 women who underwent elective caesarean sections. tissues were transported to the laboratory in pre-warmed, equilibrated leibovitz l-15 medium within 2...

Over the past 30 years many cryopreservation procedures have been applied to oocytes, embryos, sperm, ovarian and testicular tissue. Over this time many, often specialized, terms have been developed for all aspects of these procedures. This can make it difficult for readers who are not familiar with the terminology or protocols to compare and evaluate different procedures. This paper describes ...