The leader (L) proteinases of aphthoviruses (foot-and-mouth disease viruses) and equine rhinovirus serotypes 1 and 2 cleave themselves from the growing polyprotein. This cleavage occurs intramolecularly between the C terminus of the L proteinases and the N terminus of the subsequent protein VP4. The foot-and-mouth disease virus enzyme has been shown, in addition, to cleave at least one cellular...

A B S T R A C T Intrapulmonary deposition of the proteolytic enzyme papain produces a lesion resembling emphysema in experimental animals. The natural history of this lesion has not been well defined. The present study was performed to evaluate changes in lung structure and function with aging in normal rats and rats exposed to an aerosol of papain at 2 mo of age. Groups of control and papain-e...

Hydrogels are used for wound treatment, as they may contain one or more active components and protect the bed. Papain is of substances that have been with this purpose, alongside urea. In paper, carboxypolymethylene hydrogels containing papain (2% 10% concentrations) urea (5% concentration) were produced. Physical-chemical stability was performed at 0, 7, 15 30 days 2-8ºC, 25ºC 40ºC, well rheol...

The study aimed to investigate the effects of alcalase, papain, flavourzyme, and neutrase on structural characteristics bioactivity stability Cucumaria frondosa intestines ovum hydrolysates (CFHs). findings revealed that flavourzyme exhibited highest hydrolysis rate (51.88% ± 1.87%). At pH 2.0, solubility hydrolysate was lowest across all treatments, while at other levels over 60%. primary stru...

1. The u.v.-spectral characteristics of 5,5'-dithiobis-(2-nitrobenzoic acid) (Nbs(2)), 2,2'-dipyridyl disulphide (2-Py-S-S-2-Py), 4,4'-dipyridyl disulphide (4-Py-S-S-4-Py), 5-mercapto-2-nitrobenzoic acid (Nbs), 2-thiopyridone (Py-2-SH) and 4-thiopyridone (Py-4-SH) were determined over a wide range of pH and used to calculate their acid dissociation constants. 2. The reactions of l-cysteine, 2-m...

Hepatosellüler karsinom (HCC), kansere bağlı ölüm sıralamasında ikinci sırada yer alır ve dünya genelinde sıklığı artmaktadır. Papain proteolitik bir enzimdir potansiyel antikanser ajanıdır. Çalışmanın amacı, papainin insan hepatom HepG2 hücre hattındaki sitotoksik etkisinin apoptoz üzerinden değerlendirilmesidir. Papainin sitotoksisitesi MTT yöntemi ile belirlendi. uygulanan hücrelerinin morfo...