The single-cell RNA-seq allows exploring the transcriptome for one cell at a time. By doing so, cellular regulation is pictured. One limitation dropout events phenomenon, where gene observed low or moderate expression level in but not detected another. Dropouts obscure legitimate biological heterogeneity leading to description of small fraction meaningful relations. We used stochastic approach ...

Objective: The present study aims to evaluate coronavirus shedding in the tears of asymptomatic pediatric COVID-19 positive patients. Methods: A prospective interventional that included a total 145 patients hospitalized from 17 th May 2020 16 July Sohag Tropical Hospital. On admission, all them were detected through nasopharyngeal swab. They intimate contact with symptomatic before testing and ...

patients and methods five hundred and sixteen serum samples of the patients undergoing hemodialysis and kidney transplantation from various cities of khuzestan province were collected. anti-hepatitis g e2 antibodies were investigated by elisa method. rnas were extracted from serums and hepatitis g rna was detected by rt-pcr. results of the 516 samples, 38 (7.36%) specimens were positive for ant...

Background: Serology testing is an important ancillary diagnostic to the reverse transcriptase polymerase chain reaction (RT-PCR) test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We aimed evaluate performance of Roche Elecsys™ chemiluminescent immunoassay (Rotkreuz, Switzerland), that detects antibodies against SARS-CoV-2 nucleocapsid antigen, at academic laboratory in Sou...

A multiplex single-tube reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the detection and differentiation of vesicular stomatitis viruses (VSV), Indiana 1 and New Jersey, from insect samples. Using this assay, detection of either or both viruses in as little as 20 fg of total RNA from tissue culture was achieved, along with detection of vesicular stomatitis (VS) ...

The COVID-19 pandemic has underscored the shortcomings in deployment of state-of-the-art diagnostics platforms. Although several polymerase chain reaction (PCR)-based techniques have been rapidly developed to meet growing testing needs, such often need samples collected through a swab, use RNA extraction kits, and expensive thermocyclers order successfully perform test. Isothermal amplification...

Background: Rapid and accurate diagnosis of COVID-19 is critical for the management patients to limit spread infection. real-time reverse transcription polymerase chain reaction (RT- PCR) gold standard SARS-CoV-2 infection, however, it costly requires time obtain a result. A number alternative rapid tests are available now provide faster more convenient solution COVID-19. The aim this work was ...

Reverse transcriptase polymerase chain reaction (RT-PCR), virus isolation (VI) and indirect fluorescent antibody tests (IFAT) are three assays currently used by the salmon industry to identify fish infected with infectious salmon anaemia virus (ISAV). However, no data are available on the repeatability (within-laboratory consistency) and reproducibility (between-laboratory consistency) of these...

Abstract Potato viruses PLRV, PVY, PVM, PVA, PVX and PVS can cause up to 90% loss of potato harvest. Therefore, they are monitored by law in many countries using DAS-ELISA or conventional real-time RT-qPCR. Previously, we developed a multiplex DiRT-PCR (Direct reverse transcript – polymerase chain reaction), which works directly on diluted tuber sap thus saves time chemical processing for RNA e...

ObjectiveTo screen pregnant women at risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during delivery using reverse-transcription polymerase chain reaction (RT-PCR) test and serum immunoglobulin (Ig) testing.MethodBetween March 31 st August 2020, consecutive admitted for labor in a single hospital were screened SARS-CoV-2 with nasopharyngeal RT-PCR swab tests detec...