A number of Escherichia coli clones were isolated from a Lactobacillus delbrueckii subsp. lactis gene library capable of hydrolysing the chromogenic substrate Gly-Ala-beta-naphthylamide (Gly-Ala-beta NA). Some of the recombinant plasmids carried by these clones have been shown to encode the cysteine aminopeptidase gene pepC. Nucleotide sequence analyses of the plasmid inserts of the remaining c...

Expression of the C4-specific phosphoenolpyruvate carboxylase (C4-PEPC) gene in maize (Zea mays) is regulated in a tissue-specific manner, but affected by light and nutrient availability. We manipulated these stimuli in a combinatorial manner and analyzed concomitant changes in histone acetylation of the nucleosomes associated with the C4-PEPC gene in relation to transcriptional activity and st...

Skunk cabbage (Symplocarpus renifolius) blooms in early spring and its inflorescence, referred to as the spadix, can produce enough heat to melt snow. Here, we investigated glycolytic carbon flow at the PEP branch-point in thermogenic spadices. Our analyses revealed that petals and pistils in thermogenic florets exhibited higher expression of SrPEPC and SrAOX transcripts than those of SrPK, SrP...

Hereditary properties of strong growth and huge accumulation biomass in Oryza officinalis exhibit a great potential; however, the genes that code for its high photosynthesis performance are not established. This study screened eight hybrids, using analysis, based on introgression lines constructed by analyzing distant hybridization patterns between cultivars HY-8. We designed 23 primer pairs tr...

The utilization of exogenous peptides was studied in mutants of Lactococcus lactis in which combinations of the peptidase genes pepN, pepC, pepO, pepX and pepT were deleted. Multiple mutants lacking PepN, PepC, PepT plus PepX could not grow on peptides such as Leu-Gly-Gly, Gly-Phe-Leu, Leu-Gly-Pro, Ala-Pro-Leu and Gly-Leu-Gly-Leu, respectively, indicating that no other peptidases are present to...

The responses of long-term growth of plants under elevated CO2 have been studied extensively. Comparatively, the responses of plants to subambient CO2 concentrations have not been well studied. This study aims to investigate the responses of the model C3 plant, Arabidopsis thaliana, to low CO2 at the molecular level. Results showed that low CO2 dramatically decreased biomass productivity, toget...

PT Pertamina EP Cepu (PEPC) menginisiasikan Program Peningkatan Ekonomi Masyarakat melalui Optimalisasi Peran BUM Desa. Hal ini juga menjadi bentuk dukungan PEPC dalam upaya pencapaian target Pemerintah terkait pembentukan dan pengelolaan Desa di setiap desa. Tujuan utama dari program peningkatan ekonomi masyarakat optimalisasi peran adalah bagian sebuah konsep solusi terhadap dua kondisi perma...

PEPC (Pretty Efficient Parallel Coulomb-solver) is a complex HPC application developed at the Jülich Supercomputing Centre, scaling to thousands of processors. This is a case study of challenges faced when applying the Scalasca parallel performance analysis toolset to this intricate example at relatively high processor counts. The Scalasca version used in this study has been extended to disting...

Objective To translate and culturally adapt the Parental Expectations Perceptions of Children's Sibling Relationship Questionnaire (PEPC-SRQ; Kramer & Baron, 1995) into Spanish test its psychometric properties. Background relationships contribute significantly to child development; however, date, no study has validated an instrument assess quality children's sibling with samples. Method We cond...

Abstract In this study, we investigate the response of tropical cyclones (TCs) to climate change by using Princeton environment-dependent probabilistic cyclone (PepC) model and a statistical-deterministic method downscale TCs environmental conditions obtained from Geophysical Fluid Dynamics Laboratory (GFDL) High-resolution Forecast-oriented Low Ocean Resolution (HiFLOR) model, under Representa...