Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline (OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...

in published high performance liquid chromatographic (hplc) methods for analysis of topiramate (tpm) in pharmaceutical dosage forms and raw materials refractive index detector (rid) has been used which is not available in many laboratories and has low sensitivity. we described a new, sensitive and simple hplc method for determination of topiramate in pharmaceutical forms and in-virto dissolutio...

Rapid and sensitive reversed phase high performance liquid chromatography (RP-HPLC) and ultra performance liquid chromatography (RP-UPLC) method with UV detection has been developed and validated for quantification of parathyroid hormone (PTH) in presence of meta-cresol as a stabilizer in a pharmaceutical formulation. Chromatography was performed with mobile phase containing 0.1% Trifluoroaceti...

the aim of the present investigation was to biotransform the anti-inflammatory compound meloxicam by enzymes present in whole cells of five actinomycete cultures to produce novel bioactive derivatives. among the actinomycetes screened, streptomyces griseus ncim 2622 was found to possess the enzyme system(s) that oxidize meloxicam into two metabolites whereas that present in s. griseus ncim 2623...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...

A tadalafil analogue was detected in a dietary supplement marketed for tonic effect, along with hydroxyhomosildenafil and aminotadalafil. The tadalafil analogue was isolated by preparative thin layer chromatography (TLC) and its structure was elucidated using high-performance liquid chromatography (HPLC), liquid chromatography electrospray ionization-mass spectrometry (LC-ESI-MS), Fourier trans...

304 CLINICALCHEMISTRY,Vol.38, No.2, 1992 chain length on protein retention, resolution, and recovery implications for preparative HPLC. J Liq Chromatogr 1983;6:497-510. 4. Tarr GE, Crabb JW. Reverse-phase high-performance liquid chromatography of hydrophobic proteins and fragments thereof. Anal Biochem 1983;131:99-107. 5. Wu D, Walters ER. Stationary-phase ligand density effects in reversed-pha...

A rapid, sensitive and specific method for quantifying the aromatase inhibitor (anastrozole) in human plasma using dexchlorpheniramine as the internal standard (I.S.) is described herein. The analyte and the I.S. were extracted from 200 microl of human plasma by liquid-liquid extraction using a mixture of diethyl ether:dichloromethane (70:30, v/v) solution. Extracts were removed and dried in th...

Characterization of mercury binding protein in the human body is very important for understanding the metabolism and the mechanism of toxication of ingested mercuric compounds. In this study, mercury-containing protein in human plasma was separated by on-line heart-cutting two-dimensional high performance liquid chromatography (2D-HPLC). This 2D separation system used size exclusion liquid chro...

The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty...