results fifty (24.3%) of the 206 samples were culture positive. sh3 production was not detected in any of the isolates, and none of the isolated strains required co2. the results of the sensitivity test to basic fuchsin and thionin staining and specific agglutination and phage lysis (phage typing) tests indicated that all the isolated strains were biotype 1 b. melitansis. conclusions the cause ...

Streptococcus pyogenes (GAS) is a human pathogen that causes millions of infections worldwide. Comparison between GAS strains isolated in different laboratories all over the world is often difficult. Three usually used methods have either low resolution (emm typing), are expensive (MLST) or time- and labor-consuming (PFGE). Our laboratory recently developed new, inexpensive methods of GAS typin...

During a 28-month period, 5,078 cultures from a variety of anatomical sites were received for staphylococcal phage typing. Of these, 503 (10%) were not suitable for the procedure requested. Of the 4,575 viable cultures, 1,030 (23%) of the microorganisms were nontypable at both the routine test dilution (RTD) and at 100 times RTD. Of the 3,545 typable organisms, 3,061 (86%) were lysed at RTD, wh...

The nature of the phage typing reactions on Brucella species was determined by rates of adsorption and infection, one-step growth experiments, and susceptibility to lysis from without. The highest rates of adsorption and infection were obtained on smooth B. abortus cultures, and large clear plaques were produced. One or a few phage particles per B. neotomae cell killed about one-half of the cel...

Pattee, P. A. (Ohio State University, Columbus) and J. N. Baldwin. Transduction of resistance to chlortetracycline and novobiocin in Staphylococcus aureus. J. Bacteriol. 82: 875-881. 1961.-Using phage 80 of the International Typing Series propagated on appropriate strains of Staphylococcus aureus, resistance to chlortetracycline and novobiocin and the capacity to produce penicillinase were tran...

During the 1950s, Staphylococcus aureus became a major source of hospital infections and death, particularly in neonates. This situation was further complicated by the fact that Staphylococcus quickly gained resistance to most antibiotics. Controlling these infections was a pressing concern for hospital workers, especially bacteriologists who tackled it through the use of a new epidemiologic to...

A phage typing scheme for Salmonella braenderup based on both phage sensitivity and lysogenicity patterns is presented. Three S. braenderup symbiotic phages (br A, br B, and br C) were used in the phage sensitivity test, and three indicator strains were used for the lysogenicity test. The 424 strains examined were grouped in 15 of 64 possible types within this scheme. The most frequent types we...

Rhizobia are a widely distributed and abundant soil bacteria belonging to the alpha subclass of the Proteobacteria. The aim of this study was the estimation of rhizobial diversity in different genera using several molecular markers for genomic fingerprinting and the detection of certain symbiotic properties of rhizobia. Phage typing was done in order to detect different susceptibilities to atta...

A series of mutants of Escherichia coli K12 resistant to lipopolysaccharide (LPS)-specific bacteriophages were isolated, and examined with regard to their general properties, phage typing, chemical analysis of their LPS, and genetic analysis. Fourteen classes of mutants were distinguished on the basis of phage typing and sensitivity to bile salts. Three of the mutant classes are sensitive to ph...