The budding yeast Saccharomyces cerevisiae has three cell types (a cells, alpha cells, and a/alpha cells), each of which is specified by a unique combination of transcriptional regulators. This transcriptional circuit has served as an important model for understanding basic features of the combinatorial control of transcription and the specification of cell type. Here, using genome-wide chromat...

three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

Chloroplasts originate from ancient cyanobacteria-like endosymbiont. Several tens of chloroplast proteins are encoded by the chloroplast genome, while more than hundreds are encoded by the nuclear genome in plants and algae, but the exact number and identity of nuclear-encoded chloroplast proteins are still unknown. We describe here attempts to identify a large number of unidentified chloroplas...

Experimentally derived genome-wide protein interaction networks have been useful in the elucidation of functional information that is not evident from examining individual proteins but determination of these networks is complex and time consuming. To address this problem, several computational methods for predicting protein networks in novel genomes have been developed. A recent publication by ...

Annotating protein functions and linking proteins with similar functions are important in systems biology. The rapid growth rate of newly sequenced genomes calls for the development of computational methods to help experimental techniques. Phylogenetic profiling (PP) is a method that exploits the evolutionary co-occurrence pattern to identify functional related proteins. However, PP-based metho...

the current youth market is characterized as tech-savvy, variety seekers who has been active in using digital technology in unprecedented ways. the market segment defined here comprises of youth adults between the ages 20 to 30, who are more comfortable with purchasing the smartphones than previous generation. there is fierce competition in smartphone market, due to the large selection of devic...

Microorganisms play critical roles in host health. The advancement of high-throughput sequencing technology provides opportunities for a deeper understanding microbial interactions. However, due to the technological limitations 16S ribosomal RNA sequencing, microbiome data are zero-inflated, and quantitative comparison abundances cannot be made across subjects. By leveraging recent profiling te...

MOTIVATION Inferring the taxonomic profile of a microbial community from a large collection of anonymous DNA sequencing reads is a challenging task in metagenomics. Because existing methods for taxonomic profiling of metagenomes are all based on the assignment of fragmentary sequences to phylogenetic categories, the accuracy of results largely depends on fragment length. This dependence complic...

Anthropogenic stressors, such as climate change, are driving fundamental shifts in the abiotic characteristics of marine ecosystems. As the environmental aspects of our world's oceans deviate from evolved norms, of major concern is whether extant marine species possess the capacity to cope with such rapid change. In what many scientists consider the post-genomic era, tools that exploit the avai...

in this study, the relationship among four species of polygonum (including p. hyrcanicum (three samples), p. persicaria, p. avicular, and p. hydropiper) was investigated by gc profiling. furthermore, the major compounds of the ethylic ether extract of p. hyrcanicum were identified by gc/ms as: α-bisabolol (17.5%), cedrol (15.9%), sesquisabinene hydrate (13.0%), α-elemol (10.5%) and trans-longip...