BACKGROUND Neural progenitor cells (NPCs) in the developing neuroepithelium are regulated by intrinsic and extrinsic factors. There is evidence that NPCs form a self-supporting niche for cell maintenance and proliferation. However, molecular interactions and cell-cell contacts and the microenvironment within the neuroepithelium are largely unknown. We hypothesized that cellular proteases especi...

BACKGROUND In determining whether clinical decision support (CDS) should be interruptive or non-interruptive, CDS designers need more guidance to balance the potential for interruptive CDS to overburden clinicians and the potential for non-interruptive CDS to be overlooked by clinicians. OBJECTIVE (1)To compare performance achieved by clinicians using interruptive CDS versus using similar, no...

During mouse neocortical development, the Wnt-β-catenin signaling pathway plays essential roles in various phenomena including neuronal differentiation and proliferation of neural precursor cells (NPCs). Production of the appropriate number of neurons without depletion of the NPC population requires precise regulation of the balance between differentiation and maintenance of NPCs. However, the ...

Neural stem/progenitor cells (NPC) can be used for therapeutic purposes in several ways. Cultured NPCs offer a unique cell source to study the neural mechanisms in normal situations as well as in the pathogenesis of genetic diseases. In neurodegenerative disorders, potential therapies to recover the damaged neurons are NPC transplantation or activation of a patient’s own NPCs. However, the mech...

Studies in multiple sclerosis have demonstrated that normal-appearing white matter can harbor pathological changes. Here we investigated the effects of neuroinflammation, modeled by experimental autoimmune encephalomyelitis (EAE) on neural stem/progenitor cells (NPCs) located distally to inflammatory foci. We observed that EAE-derived NPCs had a lower capacity to differentiate into oligodendroc...

While much is known about the role of nuclear pore complexes (NPCs) in nucleocytoplasmic transport, the mechanism of NPC assembly into pores formed through the double lipid bilayer of the nuclear envelope is not well defined. To investigate the dynamics of NPCs, we developed a live-cell assay in the yeast Saccharomyces cerevisiae. The nucleoporin Nup49p was fused to the green fluorescent protei...

The Cutaneous Procedures Adverse Events Reporting (CAPER) Registry is a patient safety database that collects data on adverse events associated with procedures performed by dermatologists, non-dermatologist physicians, and non-physicians. Included are skin cancer surgery, laser, cosmetic procedures. A steering committee composed of board-certified clinicians, researchers from the American Socie...

Abstract Leigh syndrome (LS) is a severe manifestation of mitochondrial disease in children and currently incurable. The lack effective models hampers our understanding the mechanisms underlying neuronal pathology LS. Using patient-derived induced pluripotent stem cells CRISPR/Cas9 engineering, we developed human model LS caused by mutations complex IV assembly gene SURF1 . Single-cell RNA-sequ...