Long-term stability is a key property of enzyme membranes that can be used for biosensors, bioreactors, and bio-fuel cells. This review discusses factors that decrease the stability, and provides two examples of enzyme membranes, a polyion complex membrane and a cellulose membrane, with which stability loss can be avoided. By using these materials, long-term stability was improved. These suppor...

The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based...

Recombinant proteins can be produced in a diverse array of plant-based systems, ranging from whole plants growing in the soil to plant suspension cells growing in a fully-defined synthetic medium in a bioreactor. When the recombinant proteins are intended for medical use (plant-derived pharmaceutical proteins, PDPs) they fall under the same regulatory guidelines for manufacturing that cover dru...

This paper investigates a possible practical solution for reasonable oxygen supply during the pluripotent stem cells expansion processes, where the stem cells propagate as aggregates in stirred-suspension bioreactors. Low glucose and low oxygen concentrations are preferred for efficient proliferation of pluripotent stem cells. However, strong oxygen limitation, especially inside of cell aggrega...

SUMMARY Model based optimization can be successfully used to improve the design and operation of bioreactors. In this contribution, we present a review of this area, especially focusing on the dynamic optimization of fed-batch bioreactors, a class of problems which has attracted (and continues to receive) huge attention. Both classical and more novel approaches, like those using bio-inspired op...

Wastewater treatment based on activated sludge is known to be one of the most effective and popular wastewater purification methods. An estimation of microbial community variability in activated sludge allows us to observe the correlation between a particular bacterial group's appearance and the effectiveness of the removal of chemical substances. This research is focused on microbial community...

The use of transgenic plants for the expression of therapeutic recombinant proteins is gradually gaining acceptance in the scientific community (Moffat, 1995). There are many attractions to this technology, including the possibility of manufacture on an agricultural scale for bulk production and to reduce costs, as well as the range of plant hosts available and the stability of transgenic lines...

In this review, horizontal tubular bioreactors are discussed regarding their advantages and disadvantages compared to other bioreactor types. In horizontal tubular bioreactors medium flow is characterized by plug flow conditions that can be favorable in the case of inhibition and/or repression bioprocess kinetics. For description of liquid flow simple one-parameter or complex multi-parameters m...

To harness the potential of human pluripotent stem cells (hPSCs), an abundant supply of their progenies is required. Here, hPSC expansion as matrix-independent aggregates in suspension culture was combined with cardiomyogenic differentiation using chemical Wnt pathway modulators. A multiwell screen was scaled up to stirred Erlenmeyer flasks and subsequently to tank bioreactors, applying control...