Tomato has been a good model plant to analyze plant-pathogen interactions and its prospects for the future are promising. An international consortium named International Solanaceae Genomics Project (SOL) is proceeding with whole genome sequencing of tomato. In order to be relevant in the post-genomic era, accumulation of information on tomatopathogen interactions is important. In this review, t...

Homologues of the flavonoid 3 ,5 -hydroxylase (F3 5 H) gene, a key gene determining flower color, were obtained from a Verbena hybrida (verbena) cultivar Temari Violet, verbena cultivar Tapien Pink, and Clitoria ternatea (butterfly pea). The expression of the Temari and butterfly pea homologues in yeast confirmed that they encoded F3 5 H. The two genes under the control of an enhanced cauliflow...

large­scale consortia, highlight the increas­ ing role that pre­ competitive collaboration is assuming within biomedical research and drug development. Data­sharing projects can be completed and answer many ques­ tions that would otherwise be impossible to resolve using the data sets of each indivi­ dual company alone.” Nine more projects, including four clinical trials, are ongoing, focusing o...

In Nicotiana suaveolens N. tabacum hybrid cells incubated with high levels (0.8, 4.0 or 20 mM) of BAP at 28°C, the percentage of dead cells and extent of nuclear fragmentation were suppressed and some cultured cells remained green compared with those incubated with a standard level (0.04 mM) of BAP. Though DNA ladders were detected in all hybrid cells incubated at 28°C, positive signals for TUN...

Epigenetic regulation is defined as mechanisms that control gene expression without altering base sequences. Cytosine methylation, chromatin remodeling, and modifications at the N-termini of core histones are key factors in this regard. Epigenetic modifications are found throughout the eukaryotes, suggesting that they developed at an early stage in biological evolution, although actual molecula...

During the last few decades, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant biotechnology and their genetics studies. There are different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiates in two types first non PCR based (RFLP) and second is PCR based m...

Agrobacterium: Microbe Wiki http://microbewiki.kenyon.edu/index.php/Agrobacterium This website provides an excellent overview of the genus Agrobacterium. It contains photos and links to additional information. Genome of Agrobacterium tumefaciens http://www.genomenewsnetwork.org/articles/12_01/ A_tumefaciens_genome.shtml A. tumefaciens has been the most widely studied and used Agrobacterium spec...

To produce white-flowered gentian plants, we attempted to suppress the chalcone synthase (chs) gene by Agrobacterium-mediated transformation. A binary vector, pSMABcCHS, harboring antisense cDNA of chs isolated from the gentian Gentiana triflora cv. Maciry under control of the CaMV35S promoter was transformed into an interspecific hybrid gentian (cv. Albireo; G. scabra G. triflora). The vector ...

Tomato genome sequencing projects have started to become an internationally coordinated program. To accelerate tomato functional genomics studies in coordination with the complete sequencing of the tomato genome, we prepared a full-length cDNA library from the miniature tomato (Lycopersicon esculentum) cultivar Micro-Tom, which has attracted attention as a laboratory-grown model plant. Total RN...

Beta vulgaris L. accumulates a large amount of glycinebetaine (betaine), a metabolite that is related to salttolerance. Production of betaine involves expression of many genes that encode enzymes such as betaine aldehyde dehydrogenase, choline monooxygenase, phosphoethanolamine N-methyltransferase, S-adenosyl-L-methionine synthetase, S-adenosyl-L-homocysteine hydrolase, and methionine synthase....