Multidrug and Toxic compound Extrusion proteins (MATE) are a group of secondary active transporters with ubiquitous occurrences in all domains of life. This is a newly characterized transporter family with limited functional knowledge in plants. In this study, we functionally characterised two members of rice MATE gene family, OsMATE1 and OsMATE2 through expression in heterologous system, Arabi...

Lemnaceae plants, commonly referred to as duckweeds, are small planktonic terrestrial freshwater plants that live in symbiosis with various microbial communities. Azotobacter vinelandii typical free-living nitrogen fixing soil bacteria indirectly benefit by providing compounds. In this study, Lemna minor RDSC 5512 and A. ATCC 12837 = NBRC 13581 were co-cultured under gnotobiotic conditions. The...

DNA, in addition to the canonical B-form, can acquire a variety of alternate structures, such as G-quadruplexes. These structures have been implicated in several cellular processes in animals. In this study, we identified different types of G-quadruplex forming sequences (GQSes) in 15 sequenced plants and analyzed their distribution in various genomic features, including gene body, coding, inte...

Feasibility of Replacing Ethanolic Extract Echinophora platyloba with Antibiotic Growth Promoter in Broilers

We describe a new Renilla reniformis luciferase reporter gene, RiLUC, which was designed to allow detection of luciferase activity in studies involving Agrobacteriumbased transient expression studies. The RLUC gene was altered to contain a modified intron from the castor bean catalase gene while maintaining consensus eukaryotic splicing sites recognized by the plant spliceosome. RLUC and RiLUC ...

BACKGROUND Plant-produced biomass-degrading enzymes are promising tools for the processing of lignocellulose to fermentable sugars. A major limitation of in planta production is that high-level expression of such enzymes could potentially affect the structure and integrity of the plant cell wall and negatively influence plant growth and development. RESULTS Here, we evaluate the impact on tob...

Genistein (GEN) is a plant-derived isoflavone and can block uncontrolled cell growth in colon cancer by inhibiting the WNT signaling pathway. This study aimed to test the hypothesis that the enhanced gene expression of the WNT signaling pathway antagonist, DKK1 by genistein treatment is associated with epigenetic modifications of the gene in colon cancer cells. Genistein treatment induced a con...

Some synthetic fungicides have been currently prohibited due to their adverse effects; thus, searching for alternatives decrease application is a priority worldwide. An alternative the of chitosan -a natural biopolymer- because its biocompatibility, biodegradability, and bioactivity. Chitosan has used in different industries, such as cosmetology, pharmaceutics, food, among others. In agricultur...