To unravel the functions of cell wall and vacuolar invertases in carrot, we used an antisense technique to generate transgenic carrot plants with reduced enzyme activity. Phenotypic alterations appeared at very early stages of development; indeed, the morphology of cotyledon-stage embryos was markedly changed. At the stage at which control plantlets had two to three leaves and one primary root,...

Chinese fir (Cunninghamia lanceolata), an evergreen conifer, is the most commonly grown afforestation species in southeast China due to its rapid growth and good wood qualities. To gain a better understanding of the drought-signalling pathway and the molecular metabolic reactions involved in the drought response, we performed a genome-wide transcription analysis using RNA sequence data. In this...

We studied the effects of different media for callus induction and differentiation, and pre-culture period of immature wheat embryo culture on biolistic transformation efficiency for including antifreeze gene KN2 and bar conferring resistance to the herbicide bialaphos. The percentage of plantlets generated from induction and differentiation media without Cu2+ was lower than those cultured on d...

Mitigation of ethylene promoted leaf senescence by lysophosphatidylethanolamine (LPE) was studied. Micropropagated ‘Russet Burbank’ potato (Solanum tuberosum L.,) plantlets were grown on MS media in sterile culture tubes. After 2 weeks of growth, tubes were sealed and ethylene gas was applied to obtain 5 nL·L fi nal concentration in the culture tubes. Observations and measurements were taken tw...

Mini-cutting technique of tree species is an effective method maintaining desirable features plant matrices and uniformity. The objective the present research was to determine substrate concentration indolebutyric acid (IBA) more suitable for mini-cuttings Carya illinoinensis rooting. experiment carried out in a greenhouse with mist, from February May 2018. used experimental design blocks facto...

ABSTRACT: The banana tree (Musa spp.) is the second most produced fruit crop and first consumed in Brazil. Among constraints affecting causing production losses are plant-parasitic nematodes. objective of present work was to evaluate effect neem (Azadirachta indica), Annona muricata (soursop) A. squamosa (sugar apple) aqueous leaf extracts treatment plantlets cv. Comprida, naturally infected by...

the purpose of this study was to examine the effectiveness of nanosilver colloid as a new antimicrobial agent against erwinia in tissue culture of sugar beet for the recovery of the contaminated doubled haploid plantlets. the nanosilver colloidal solution of 4000 ppm was diluted in different concentrations (0, 50, 75, 100 and 125 ppm) and was added to the solid ms medium. in a factorial experim...

A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environment. The greenhouse and field have substantially lower relative humidity, higher light level and septic environment that are stressful to micropropagated plants compared to in vitro conditions. The benefit of any micropropagation system can, however, only be fully reali...

<p><em>Sugarcane productivity included cane yield and sugar extracted rate (rendemen) fluctuated annually. The main causes of low sugarcane is quality planting material with no high (sugar rendemen) potential, resistance to pests diseases, stability production, tolerance climate changed early age harvest. For increasing the producing a superior competitiveness cost, vegetative propa...

Production of hybrid Phalaenopsis seedlings is generally applied by in vitro culture techniques. The final stage acclimatization. acclimatization crucial because the plantlets must adapt to ex-vitro environment. Shade supports plantlet growth during early stages This study aims determine effect shade level on physiological and anatomical characteristics orchid at stage. research used shading le...