BACKGROUND Diagnosis of pheochromocytoma (PC) is based on a combination of clinical suspicion, finding an adrenal mass, increased plasma, and urine concentrations of catecholamine metabolites and is finally confirmed with histopathology. In human medicine, it is controversial whether biochemically testing plasma is superior to testing urine. OBJECTIVES To measure urinary and plasma catecholam...

capture elisa system was developed for diagnosis of visceral leishmaniasis (vl) using a monoclonal antibody raised against an antigen previously detected in the urine of vl patients. urine samples from confirmed vl cases from yemen, nepal, spain, sudan and brazil were tested in the capture elisa in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

PURPOSE Prostate specific antigen (PSA) and human glandular kallikrein (hK2) are mainly produced by the prostate and their genes are regulated by androgens through the androgen receptor. We determine whether PSA and hK2 change significantly in plasma and urine after antiandrogen treatment in male-to-female transsexuals. MATERIALS AND METHODS Plasma and urine PSA and hK2 were measured with hig...

BACKGROUND Pheochromocytomas and paragangliomas (PPGLs) are potentially lethal yet usually surgically curable causes of endocrine hypertension; therefore, once clinical suspicion is aroused it is imperative that clinicians choose the most appropriate laboratory tests to identify the tumors. CONTENT Compelling evidence now indicates that initial screening for PPGLs should include measurements ...

A method is described for the measurement of magnesium in biological materials and has been applied to the Unicam flame spectrophotometer SP 900. Samples were diluted in 10 mM strontium ethylenedinitrolo tetraacetate (EDTASr), 10 mM potassium carbonate, giving final magnesium concentrations of about 0.1 to 0.2 mM. The EDTASr almost eliminated the depression of magnesium emission by interfering ...

Gas chromatography-mass spectrometry quantitative method was developed to monitor concentrations of methadone and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in plasma and urine of patients. The developed method was simple, accurate and reproducible to quantify methadone and EDDP in plasma and urine samples in the concentration range of 15-1,000 and 50-2,000 ng/mL, r...

A high-performance liquid chromatographic technique for the simultaneous determination of prednisolone and prednisone in human plasma, whole blood, urine, and bound-to-plasma proteins, using betamethasone as internal standard, is presented. Liquid-liquid extraction is used for whole blood samples, and solid phase extraction is used for plasma, urine, and proteins bound to plasma. The accuracy, ...

      A new method was developed for the spectral resolution by further determination of three- and  four-component  mixtures  of  drugs in urine samples through  the complementary application of multivariate curve resolution-alternating least squares with correlation  constraint. In the current study, a simple method was proposed to construct a calibration set for the mixture of drugs in the p...

PURPOSE This research examines the profile of metabolites of thalidomide that are formed in refractory multiple myeloma patients undergoing thalidomide therapy in comparison with those that are detected in healthy mice. EXPERIMENTAL DESIGN Urine or plasma samples from patients during thalidomide therapy (100-400 mg daily), or from mice treated i.p. (100 mg/kg) or p.o. with thalidomide (50 mg/...