It has been reported that diverse treatments which depolarize the plasma membrane of Neurospora crassa produce rapid increases in cyclic adenosine 3',5'-monophosphate (cyclic AMP) levels. In the current study, membrane active antibiotics, which are known or putative depolarizing agents, were found to produce similar cyclic AMP increases, not only in N. crassa, but also in the distantly related ...

The present study was conducted to find the effect of three heavy metals, Ag, Hg and Pb on the expression level of a gene encoding plasma membrane H+-ATPase in Aeluropus littoralis. The experiment was laid out in a completely random design with three replications. The expression of the main gene was normalized to the expression of the housekeeping gene actin. Two 259 and 187 bp fragments were a...

Abstract The study was executed to investigate the different cooling periods (0, 24, and 48 hours) effects on plasma membrane integrity depending hypo osmotic swelling test (HOST), spermatozoa motility, acrosome integrity, live sperm, abnormalities, correlation among Host other rams sperm parameters This done at an animal farm in Al-Kut city (southern Baghdad city\ Iraq), from 5th January Febru...

The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5oC and frozen-thawed. A conventional hypo-osmotic swelling t...

evidence supports the involvement of nitric oxide (no) in a varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. however, low concentration of no is essential in biology and physiology of spermatozoa, but high amounts of no is toxic and has negative effects on sperm functions. on the other hand, it is established t...

Physically distinct cholesterol/sphingolipid-rich plasma membrane microdomains, so-called lipid rafts, have been recognized to play an important regulatory role in various cellular processes, from membrane trafficking to signal transduction, in a number of cell types. We report here that the ability of TCR on activated, functional CD8+ T lymphocytes to efficiently bind MHC class I tetramer comp...

Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways death; (2) method to measure using lactate dehydrogenase (LDH) release as marker membrane integrity; (3) flow cytometry that simultaneously measures two types death, necrosis apoptosis; (4) use fluorescence microscopy nuclear morphology ...