This study investigated the effects of the size of chromosomal DNA fragments on transformation efficacy of a plasmid. In our experiment, each of three chromosomal fragment sizes were co-transformed with varying amounts of plasmid DNA and results show that the conditions, plasmid alone, plasmid with 10kb, and plasmid with 20kb fragments gave no significant differences. On the other hand, plasmid...

Efficient initiation from ammonium trifluoroacetate salts (TFA) dendritic end-groups yields well-defined poly( l -lysine) star polypeptides with 8, 16 and 32 arms. Hydrolytic core degradation plasmid DNA complexation is demonstrated.

conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

conclusions resistance to oxacillin and other antibiotics was high, and most of the isolates were found to be multi-drug resistance (mdr). plasmid analysis of representative s. aureus isolates also demonstrates the presence of a wide range of plasmid sizes, with no consistent relationship between plasmid profiles and resistance phenotypes. regular surveillance of hospital infections and monitor...

objective: il18 is a cytokine that plays an important role in the t-cell-helper type 1 (th1) response and hence, plasmid-encoded il18 is considered as a potent genetic adjuvant for dna vaccine studies. in this study, a bicistronic eukaryotic plasmid capable of secreting a more stable mouse il18 (fused with fcγ2a fragment) was constructed and expression of this chimer cytokine was also assessed....

Plasmid genotypes of root nodulating rhizobial isolates of Sesbania, sampled from six ecologically distinct habitats, were characterized. Plasmid profile analysis revealed nine different plasmid types having molecular masses ranging from 30 to 300 MDa, distributed among six profile types that grouped the isolates into six plasmid classes. The six plasmid profiles were diverged from each other a...

Introduction: Cervical cancer is the second most common cancer in women worldwide and the role of human papillomavirus (HPV) has been proved in its etiology. The currentley available L1-capsid-protein-based vaccine is highly immunogenic and very high titers of serum antibodies can be obtained by its injection, but unfortunately it is restricted to only a few HPV genotypes and is relatively expe...