Mutations in the QUARTET loci in Arabidopsis result in failure of microspore separation during pollen development due to a defect in degradation of the pollen mother cell wall during late stages of pollen development. Mutations in a new locus required for microspore separation, QRT3, were isolated, and the corresponding gene was cloned by T-DNA tagging. QRT3 encodes a protein that is approximat...

Enzymically active cell wall from ripe tomato (Lycopersicon esculentum Mill.) fruit pericarp release uronic acids through the action of wall-bound polygalacturonase. The potential involvement of products of wall hydrolysis in the induction of ethylene synthesis during tomato ripening was investigated by vacuum infiltrating preclimacteric (green) fruit with solutions containing pectin fragments ...

Yeast polygalacturonase (YPG) is a constitutive type enzyme, produced exocellularly and free of pectinesterase by Saccharomyces jragilis (1). It has an optimal pH of 4.4 and appears to be specific in its activity towards polygalacturonides (2). YPG differs from preparations of commercial fungus polygalacturonase in that it cannot carry out a complete breakdown of pectic acid to galacturonic aci...

Vegetable proteins such as soybean meal, canola meal and lupins are widely used as the main protein source in poultry diets. However, there is only limited information on the nutritive or anti–nutritive effects of indigestible non–starch polysaccharides in vegetable proteins. This review discusses a number of experiments which investigated the effects of feed enzymes on the utilisation of non–s...

Fruit softening is an important part of the ripening process, and involves changes to cell turgor and primary cell wall structure. Most of the polysaccharide components of the cell wall are subjected to some degree of controlled degradation, resulting in a loosening and swelling of the wall structure, a weakening of cell wall strength, and reduced intercellular adhesion. Early ripening changes ...

BACKGROUND Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has...

The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...