The emergence of multidrug-resistant bacteria has necessitated the search for novel bioactive compounds in unexplored and natural environments. Actinobacteria contain important substances. are Gram-positive, facultatively anaerobic, filamentous, fungus-like that remain among leading producers antibiotics. Consequently, this work, 50 actinobacteria were isolated from 5 soil samples collected […]

Little is known about the impacts of transgenic plants and micro-organisms on soil biota and processes, despite the large number of field releases of transgenic crops. Recent work has shown that transgenic plants can cause changes in the soil biota (both invertebrates and micro-organisms) associated with these plants. Often the changes are transient but their impact on the soil ecosystem remain...

Heavy metal pollution by natural factors is a world-wide phenomenon. Release of large quantities of heavy metals without handling proper processes that could decrease the concentration of such a material is a hassle that makes strains resistant to these heavy metals apart from entering into human food chain. In this research, wastewater of four firms in Guilan province such as Foolad, Risandegi...

Burkholderia pseudomallei (Bp), the causative agent of melioidosis, is unevenly distributed in the complex soil environment. Physicochemical factors in the soil have been reported to affect microbial communities in the soil. The effect of physicochemical factors on the number and diversity of organisms in the soil has not been reported. Twenty-five each B. pseudomallei-positive and -negative so...

Direct counting techniques, first developed for aquatic samples, can be used to enumerate bacteria in soil and groundwater sediments. Two fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) for actively respiring bacteria and 4(prm1),6-diamidino-2-phenylindole (DAPI) for total bacteria, were tested for their usefulness in epifluorescent direct bacterial enumeration in soil. Both dy...

The effects of heavy metals and phytoextraction practices on a soil microbial community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulescens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bacteria and the beta-Proteobacteria and the amoA gene (encoding the alpha-subunit of ammonia monooxygenase) were PCR-amplified and analysed by d...

Phylogenetic composition of bacterial community in soil of a karst forest was analyzed by culture-independent molecular approach. The bacterial 16S rRNA gene was amplified directly from soil DNA and cloned to generate a library. After screening the clone library by RFLP, 16S rRNA genes of representative clones were sequenced and the bacterial community was analyzed phylogenetically. The 16S rRN...