نتایج جستجو برای: premature translation

تعداد نتایج: 189794  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علامه طباطبایی - دانشکده ادبیات و زبانهای خارجی 1389

نمایشنامه به پیروی از ماهیت دوگانه اش، کارکردی دوگانه به خود می گیرد، بدین معنا که در هر دو نظام ادبی و تئاتری می تواند کارکردی مختص به خود یابد. هدف از انجام این پژوهش یافتن تفاوت ها میان ترجمه صفحه و ترجمه صحنه است.. پرسش مطرح شده عبارتست از: بر اساس مدل ارائه شده توسط لدوسر (1995)، تفاوت های ترجمه صفحه و ترجمه صحنه در چیست؟ یافته های این پژوهش حاکی از آن است که ترجمه صفحه به نمایشنامه ا...

2012
Yuka Adachi Hiroshi Kuroda Yasushi Yukawa Masahiro Sugiura

The chloroplast psbD and psbC genes encode the D2 and CP43 proteins of the photosystem II complex, and they are generally cotranscribed. We report studies on the basic translation process of tobacco psbD-psbC mRNAs using an in vitro translation system from tobacco chloroplasts. The primary transcript has an unusually long 5'-UTR (905 nt). We show that it is translatable. Processing of the 5'-UT...

2013
Johann W. Bauer Clemens Brandl Olaf Haubenreisser Bjoern Wimmer Manuela Weber Thomas Karl Alfred Klausegger Michael Breitenbach Helmut Hintner Tobias von der Haar Mick F. Tuite Lore Breitenbach-Koller

Evidence is now accumulating that sub-populations of ribosomes - so-called specialized ribosomes - can favour the translation of subsets of mRNAs. Here we use a large collection of diploid yeast strains, each deficient in one or other copy of the set of ribosomal protein (RP) genes, to generate eukaryotic cells carrying distinct populations of altered 'specialized' ribosomes. We show by compara...

Journal: :Journal of immunology 1999
J A Ragheb M Deen R H Schwartz

To investigate the role that translation plays in the stabilization of the IL-2 mRNA, we inhibited protein synthesis in both cis and trans. To block translation in trans, we utilized the inhibitors puromycin (PUR) and cycloheximide (CHX), which differentially effect polysome structure. We found that CHX enhances the stability of IL-2 mRNA in cells stimulated with anti-TCR Ab alone, but it inhib...

2014
Krystyna Mazan-Mamczarz X. Frank Zhao Bojie Dai James J. Steinhardt Raymond J. Peroutka Kimberly L. Berk Ari L. Landon Mariola Sadowska Yongqing Zhang Elin Lehrmann Kevin G. Becker Rita Shaknovich Zhenqiu Liu Ronald B. Gartenhaus

Deregulation of the translational machinery is emerging as a critical contributor to cancer development. The contribution of microRNAs in translational gene control has been established however; the role of microRNAs in disrupting the cap-dependent translation regulation complex has not been previously described. Here, we established that elevated miR-520c-3p represses global translation, cell ...

Journal: :The EMBO journal 2011
Guang Zhao Wei Kong Natasha Weatherspoon-Griffin Josephine Clark-Curtiss Yixin Shi

We have characterized a 17-residue peptide, MgtL, which is translated specifically in high Mg(2+) from an open reading frame (ORF) embedded in the Mg(2+) riboswitch domain, previously identified in the 5' leader region of Mg(2+) transporter gene mgtA in Salmonella. We demonstrate that mgtL translation is required to prematurely terminate mgtA transcription. Abrogation of mgtL translation by mut...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2007
Mikiei Tanaka P Boon Chock Earl R Stadtman

To investigate the effect of RNA oxidation on normal cellular functions, we studied the translation of nonoxidized and oxidized luciferase mRNA in both rabbit reticulocyte lysate and human HEK293 cells. When HEK293 cells transfected with nonoxidized mRNA encoding the firefly luciferase protein were cultured in the presence of paraquat, there was a paraquat concentration-dependent increase in th...

Journal: :RNA 2010
Isabel S Naarmann Christiane Harnisch Gerhard Müller-Newen Henning Urlaub Antje Ostareck-Lederer Dirk H Ostareck

Erythroid precursor cells lose the capacity for mRNA synthesis due to exclusion of the nucleus during maturation. Therefore, the stability and translation of mRNAs that code for specific proteins, which function in late stages of maturation when reticulocytes become erythrocytes, are controlled tightly. Reticulocyte 15-lipoxygenase (r15-LOX) initiates the breakdown of mitochondria in mature ret...

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