Background: High-quality oligonucleotides for molecular amplification and detection procedures of diverse target sequences depend on sequence homology. Processing input identifying homogeneous regions in alignments can be carried out by hand only if they are small contain high similarity. Finding the best large inhomogeneous needs to automated. Results: The ConsensusPrime pipeline was developed...

When a family of genes from closely related organisms is known, there is a certain change to extract the corresponding gene from the genome of another related organism. This can be done by polymerase chain reaction, provided that a pair of suitable primers can be designed. In contrast to primer design for a single, known target sequence, systematic primer design for an unknown target given a gr...