Synthesis of a natural proline-rich cyclopolypeptide - rolloamide A [8] was carried out by coupling of tri- and tetrapeptide units Boc-Phe-Pro-Val-OMe and Boc-Pro-Leu-Pro-Ile-OMe after proper deprotection at carboxyl and amino terminals using carbodiimide chemistry in alkaline environment followed by cyclization of linear heptapeptide segment in the presence of base. The structure of synthesize...

Streptococcus mutans is considered to be the major etiologic agent of human dental caries. Attachment of S. mutans to the tooth surface is required for the development of caries and is mediated, in part, by the 185-kDa surface protein variously known as antigen I/II, PAc, and P1. Such proteins are expressed by nearly all species of oral streptococci. Characteristics of P1 include an alanine-ric...

Abbreviations. AGP, arabinogalactan protein; EGFP, enhanced green fluorescent protein; GPI, glycosylphosphatidylinositol; GAGP, gum arabic glycoprotein; GT, glycosyltransferase; HRGP, hydroxyproline rich-glycoprotein; LeAGP1, arabinogalactan protein 1 from tomato; Lys, lysine rich domain; P4H, prolyl-4-hydroxylase; (SP), serine-proline repeat; (VP), valine-proline repeats; (TP), threonine-proli...

Indolicidin is a cationic, 13-residue antimicrobial peptide (ILPWKWPWWPWRR-NH(2)) which is unusually rich in tryptophan and proline. Its antimicrobial action involves the bacterial cytoplasmic membrane. Fluorescence and circular dichroism spectra demonstrated the structural similarity of indolicidin in complexes with large unilamellar phospolipid vesicles and with detergent micelles. The struct...

Numerous plant species have been known for decades that respond to herbivore attacks by systemically synthesizing defensive chemicals to protect themselves from predators. The nature of systemic wound signals remained obscure until 1991, when an 18-aa peptide called systemin was isolated from tomato leaves and shown to be a primary signal for systemic defense. More recently, two new hydroxyprol...

In present study a proline rich protein has been identified in lentil EST database using (soyabean) glycine max proline rich protein sequence as query sequence known to be down regulated in response to salinity and drought stress. Sequence similarity search has shown 90 % similarity with chickpea EST sequence Accesion no. GR 398344 and 85% similarity with Lens culinaris EST sequence Accession n...

Cell adhesion kinase beta (CAKbeta) is a protein tyrosine kinase closely related to focal adhesion kinase (FAK) in structure. CAKbeta contains two proline-rich sequences within its C-terminal region. Since proline-rich sequences present in the corresponding region of FAK are known to mediate protein-protein interactions by binding to SH3 domains, we investigated binding of CAKbeta to a panel of...

The development of antimicrobial peptides as new class of antibiotic agents requires structural characterisation and understanding of their diverse mechanisms of action. As the cyclic hexapeptide cWFW (cyclo(RRRWFW)) does not exert its rapid cell killing activity by membrane permeabilisation, in this study we investigated alternative mechanisms of action, such as peptide translocation into the ...

Synthesis of a natural proline-rich cyclopolypeptide - rolloamide A [8] was carried out by coupling of tri- and tetrapeptide units Boc-Phe-Pro-Val-OMe and Boc-Pro-Leu-Pro-Ile-OMe after proper deprotection at carboxyl and amino terminals using carbodiimide chemistry in alkaline environment followed by cyclization of linear heptapeptide segment in the presence of base. The structure of synthesize...

A conformation of the neurohypophyseal hormone oxytocin in solution is proposed. The structure possesses, in addition to the beta-turn comprised of the sequence -L-tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl- in the ring component of the hormonal molecule, a second beta-turn involving the C-terminal oxytocin sequence, -L-cysteinyl-L-prolyl-L-leucylglycinamide. The resulting oxytocin structur...