We have investigated the production of prophage deletions in heat-induced lysogens of E . coli K12. Our results are indicative of a direct action of the heat-induced prophage in producing deletions. The temperature of 40" used for the experiments may be critical to prove this effect. The phage function involved in deletion formation is not known. H E A T treatment of a K12 lysogen carrying a CZ...

In a thermosensitive dnaZ mutant lysogenic for lambda, induction of the prophage development was provoked at the restrictive temperature. In dnaD strain, spontaneous release of lambda proceeded even at 43 degrees C, but distinct induction of the prophage development was not caused by a heat treatment. Similarly, shift up of growth temperature did not lead to induction of lambda in lysogens carr...

Salmonella enterica is a bacterial species that is a major cause of illness in humans and food-producing animals. S. enterica exhibits considerable inter-serovar diversity, as evidenced by the large number of host adapted serovars that have been identified. The development of methods to assess genome diversity in S. enterica will help to further define the limits of diversity in this foodborne ...

A series of independent Escherichia coli K eductants has been isolated and tested to determine the extent of their deletions. The deletions cover the P2 prophage in location H, the his operon, a suppressor of the recBC phenotype (sbcB), the gene for gluconate-6-phosphate dehydrogenase (gnd), a locus involved in cell wall synthesis (rfb), and in some cases all or part of genes involved in methyl...

multiplication of T5. This Sh(P2). The inhibition is clearly a function of blockage must occur later on in the latent petwo factors-the prophage and the carrier cell. riod since adsorptionof phageand the first stage of Inhibition of PB multiplication does not occur, infection takes place. The presence of P2 prophage and it is evident that this difference between the in strains of E. coli, howev...

Cronobacter sakazakii infection is particularly harmful to infants, and putative virulence factors of prophage origin have been identified in C. sakazakii. In this study, the phage ENT39118 was isolated from wild-type C. sakazakii; it belongs to the family Siphoviridae. The genomic sequence of phage ENT39118 was composed of circular double-stranded DNA with a length of 39,012 bp. The sequence o...

To establish a lysogenic lifestyle, the temperate bacteriophage φC31 integrates its genome into the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment site in the phage DNA) and attB (the chromosomal attachment site). This reaction is promoted by a phage-encoded serine recombinase Int. To return to the lytic lifestyle, the prophage excises its DNA b...

Background Most bacterial genomes contain integrated bacteriophages—prophages—in various states of decay. Many are active and able to excise from the genome replicate, while others cryptic prophages, remnants their former selves. Over last two decades, many computational tools have been developed identify prophage components genomes, it is a particularly area for applicati...