The neutral protease of Bacillus polymyxa had a broad pH optimum (6.0 to 7.2) for activity at 37 C. The enzyme was most stable at pH 5.6 to 5.8. The protease had an optimum temperature of 37 C and was quite thermostable up to 35 C, but at higher temperatures the stability decreased rapidly. The substrate specificity of the protease was similar to that of the neutral proteases of other members o...

In order to reveal the effect of exogenous neutral protease addition on quality cigar tobacco leaves during fermentation. Taking Sichuan Shifang as research object, changes conventional chemical composition, amino acid content, organic acids and other substances in after natural fermentation with enzyme preparations were investigated, sensory before also investigated. The results show that: 1) ...

1. The cartilage-proteoglycan-degrading activity of synovial fluid cells from rheumatoid patients is primarily due to neutral protease activity; hyaluronidase does not contribute significantly. Evidence for these conclusions is adduced from electrophoresis of degradation products, from comparison of the proteoglycan-degrading and protease activities of extracts and from the effects of activator...

Streptomyces griseus var. alcalophilus was selected because it secreted a unique protease (protease I) that catalyzed the transfer reaction forming the hydroxamic acids of various amino acids. Protease I was purified to the electrophoretically homogeneous state and an activity of more than 125-fold that of the culture broth. The molecular weight of the enzyme was estimated to be 25,000 by gel f...

Actinomycetes is one of the microorganisms which have ability enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer hydrolase can be used a candidate probiotic. A probiotic should good resistance in different environmental conditions including pH salinity.  This research conducted to filter candidate’s probiotics from bacteria that tolerance salinity has activity ...

The Bowman-Birk protease inhibitor (BBI) has been shown to be an effective suppressor of carcinogenesis in vivo and in vitro. To elucidate the mechanism(s) by which BBI suppresses carcinogenesis, we believe it will be necessary to identify and characterize the target enzymes that specifically interact with the BBI. We have shown previously that several cellular proteins in C3H/10T1/2 mouse embr...

Biochemical and enzymatic characterization for extracellular protease isolated from Cordyceps militaris cultivated on rice bran medium was investigated. C militaris produced proteolytic enzymes from 10 days after inoculation, maximum enzyme production was found at 25 days. The optimum temperature and pH of proteases production was at 25C and pH 7.0, respectively. The protease activity was obser...

Quality feed in aquaculture is very important to increase the growth and health of fish. Low efficiency can be overcome by supplementation using catalytic enzymes. One enzymes that stimulate digestion protease red ginger plants. The enzyme plants plays a role breaking peptide bonds place carboxyl groups so peptides are formed from complex compounds into simpler compounds. This study aims determ...

Abstract Proteases derived from human pathogens can specifically cleave IgG into F(ab’)2 and Fc fragments. IdeS, an cleaving enzyme Streptococcus pyogenes, has shown clinical proof of concept, is approved for use before kidney transplantation. Due to the immunogenic nature these proteases, dosing limited by high prevalence pre-existing antibodies induction anti-drug after dosing. Therefore, mit...

In this report we describe a novel chymotrypsin-like serine protease produced by Streptomyces griseus. The enzyme has been tentatively named S. griseus protease C (SGPC). The gene encoding the enzyme (sprC) was identified and isolated on the basis of its homology to the previously characterized S. griseus protease B (SGPB). The sprC gene encodes a 457-amino acid prepro-mature protein of which o...