A new protease which specifically acts on the apo-form of pyridoxal enzymes was discovered in rat small intestine and skeletal muscle. This enzyme was puriCed about SM-fold from the small intestine of vitamin Be-deficient rats. Some proporties of the enzyme and its action were studied. The enzyme split the apo-protein of ornithine transaminase into two products, a homogeneous smaller protein an...

Proteases, though essentially indispensable to the maintenance and survival of their host organisms, can be potentially damaging when overexpressed or present in higher concentrations, and their activities need to be correctly regulated. An important means of regulation involves modulation of their activities through interaction with substances, mostly proteins, called protease inhibitors. Some...

Dichelobacter nodosus is the principal causative agent of ovine footrot and its extracellular proteases are major virulence factors. Virulent isolates of D. nodosus secrete three subtilisin-like serine proteases: AprV2, AprV5 and BprV. These enzymes are each synthesized as precursor molecules that include a signal (pre-) peptide, a pro-peptide and a C-terminal extension, which are processed to ...

The production of protease by isolated aleurone layers of barley in response to gibberellic acid has been examined. The protease arises in the aleurone layer and is mostly released from the aleurone cells. The courses of release of amylase and protease from aleurone layers, the dose responses to gibberellic acid and the effects of inhibitors on the production of both enzymes are parallel. As is...

Proteases are one of the most industrially important enzymes, which account for about 60% total enzyme market. Protease production by submerged fermentation in shake flasks using Bacillus sp. isolated from soil was studied. Soil samples were collected different locations within Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra state. The serially diluted and inoculated on sterilized skim mil...

Thermal bacteria that live in higher temperature have been considered as good candidates for bioremediation and processing of protein-rich wastewater. However, very little is known about the proteases, the enzymes that digest the protein wastes in these organisms. In this study, we present a comparative genomic analysis of the protease complement in a thermal bacterium Coprothermobacter proteol...

OBJECTIVE This study evaluated the change and function of the pancreas, and small intestine in relation to growth performance of broilers on diets supplemented with raw soybean meal (RSBM) and protease. Samples of test ingredients and diets, after mixing and prior to being used were also assessed on contents of anti-nutritional factors. METHODS A 3×3 factorial study was used, with three level...

BACKGROUND AND OBJECTIVES Proteases are a group of enzymes that catalyze the degradation of proteins resulting in the production of their amino acid constituents. They are the most important group of industrial enzymes which account for about 60% of total enzymes in the market and produced mainly by microorganisms. The attempts were made to study the kinetic parameters of protease produced by S...

OBJECTIVES To assess the prevalence of enzyme sensitisation in the animal feed industry. METHODS A cross sectional study was conducted in four animal feed factories, where several enzymes had been used in powder form for 7-9 years. Before this study, enzymes in liquid form had started to be used. Sensitisation to enzymes was examined by skin prick and radioallergosorbent (RAST) tests. Altoget...