For all organisms promoting protein homeostasis is a high priority in order to optimize cellular functions and resources. However, there is accumulating evidence that aging leads to a collapse in protein homeostasis and widespread non-disease protein aggregation. This review examines these findings and discusses the potential causes and consequences of this physiological aggregation with age in...

background: recombinant proteins overexpressed in e. coli are usually deposited in inclusion bodies. cysteines in the protein contribute to this process. inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed in e. coli. hence, aggregated proteins should be solubilized and allowed to refold to obtain nat...

Background and Aims: Coated nanoparticles have different surface chemistry, aggregation, and interaction properties. The aim of this study was to investigate the cytotoxicity of silver nanoparticles AgNPs coated with different proteins on Balb/c macrophages. Materials and Methods: In this study these items were evaluated: 1) the size of aggregation, 2) the quantity and mechanisms of uptake, ...

1 7 6 7 jci.org Volume 125 Number 5 May 2015 Introduction Amyotrophic lateral sclerosis (ALS) is a progressive, fatal disorder of motor neurons that begins focally and spreads, leading to weakness of limb, respiratory, and bulbar muscles. Immediately preceding death, there is a near total loss of limb and respiratory function, as well as a loss of the ability to chew, swallow, and speak. ALS is...

Protein aggregation is associated with age-related neurodegenerative disorders, such as Alzheimer's and polyglutamine diseases. As a causal relationship between protein aggregation and neurodegeneration remains elusive, understanding the cellular mechanisms regulating protein aggregation will help develop future treatments. To identify such mechanisms, we conducted a forward genetic screen in a...

Chapter Outline Introduction 105 Multi-Parameter Microscopy of Protein Aggregation Kinetics 106 Fusion Protein Labeling of Amyloidogenic Proteins 106 Principle of Multi-Parameter Imaging Techniques 107 Fluorescence Anisotropy Imaging 107 Principle of HomoFRET in the Study of Protein SelfAssembly Reactions 107 HomoFRET Imaging for the Study of α-synuclein Aggregation 108 Fluorescence Lifetime Im...

Protein aggregation is a widespread phenomenon in cells and associated with pathological conditions. Yet, little is known about the rules that govern protein aggregation in living cells. In this study, we biochemically isolated aggregation-prone proteins and used computational analyses to identify characteristics that are linked to physiological and arsenite-induced aggregation in living yeast ...