xanthomonas citri subsp citri (xcc) is responsible for one of the most destructive diseases of citrus fruits, asiatic citrus canker. based on the bacterial host range, the pathogenic forms, a, a*, and aw have been identified. a*-type strains are not well characterized and they have been reported to differ from other a-type strains on the basis of biochemical and genetic characteristics. in this...

The surfactant precipitation and recovery method (SPR method) consist of a step, that is protein-surfactant complex by the addition aqueous solution to protein solution, dissolution precipitate in polar organic solvent trace amount salt solution. When denaturant was not included, ratio lysozyme increased as AOT/lysozyme molar increased, after reaching 100%, decreased due redissolution precipita...

We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone. We also describe protein solubilization prior to IEF, either in classical rod gels or in IPGs, using two different solutions. The procedure is easy to carry out. The major caveats are...

A virus-free soluble antigen, obtained by ammonium sulfate precipitation of the supernatant fluids of La Crosse virus-infected BHK-21 cell cultures, was more reactive and more specific than infected suckling mouse brain antigen when compared by immunodiffusion and counterelectrophoresis tests. By complement fixation tests, the antigen was cross-reactive with heterologous California group arbovi...

A protein was isolated from bovine thymus that was shown to accelerate the appearance of hemolysin to sheep erythrocytes in neonatal mice. The isolation procedure consisted of saline homogenization, ammonium sulfate precipitation, and methanol precipitation, followed by chromatography on diethylaminoethyl-cellulose and two chromatographies on Sephadex G-150. The protein was shown to be homogene...