An incompletely branched, low molecular weight form of glycogen has been isolated from liver tissue of a patient with glycogen storage disease. Digestion with pullulanase and @-amylase, oxidation by periodate, Smith degradation, spectral studies of the iodine complexes, osmotic pressure measurements, and end group analysis gave results which indicate a molecular weight range of 8,000 to 15,000 ...

The aim of this work was to identify enzymes that participate in the degradation of transitory starch in Arabidopsis. A mutant line was isolated by screening leaves at the end of the night for the presence of starch. The mutant had a higher starch content than the wild-type throughout the diurnal cycle. This accumulation was due to a reduction in starch breakdown, leading to an imbalance betwee...

The maltose regulon (mal regulon) has previously been shown to consist of the mal gene cluster (malMP, malXCD and malAR operons) in Streptococcus pneumoniae. In this study, we have further elucidated the complete mal regulon in S. pneumoniae D39 using microarray analyses and β-galactosidase assays. In addition to the mal gene cluster, the complete mal regulon of S. pneumoniae D39 consists of a ...

Pullulanase from Bacillus acidopullulyticus was purified on a packed bed and a fluidized bed of calcium alginate beads. The binding of enzyme activity to the medium was found to follow Langmuir isotherm pattern. The maximum binding capacity was 1476 U ml(-1) matrix and the dissociation constant was 142 U ml(-1). The dynamic binding capacities at 5% breakthrough in the packed and fluidized beds ...

In this work, linear dextrins (LDs) with the fragment F-40 (DP = 31.44) were fabricated from waxy potato starch through pasteurization and enzymatic debranching by pullulanase then separated extracted ethanol solutions different concentrations. The LDs used to encapsulate hydrophobic ligand curcumin develop a controlled release system that would increase its flavor in food functions medicines. ...