Tetrahydrofolates (THF) are a family of cofactors that function as one-carbon donors in folate-dependent one-carbon metabolism, a metabolic network required for the de novo synthesis of purines, thymidylate, and for the remethylation of homocysteine to methionine in the cytoplasm. 5-FormylTHF is not a cofactor in one-carbon metabolism, but serves as a storage form of THF cofactors. 5-formylTHF ...

Acute myeloid leukemia (AML) is a heterogeneous disease with poor clinical outcomes. We have previously shown that constitutive activation of NADPH oxidase 2 (NOX2), resulting in over-production reactive oxygen species (ROS), occurs over 60% AML patients. also increased ROS production promotes glucose uptake and proliferation cells, mediated by changes carbohydrate metabolism. Given carbohydrat...

According to studies, the microbiome may contribute emergence and spread of breast cancer. E. coli is one Enterobacteriaceae family recently found be present as part tissue microbiota. In this study, we focused on effect secretome free cells MCF-7 metabolism. Liquid chromatography–mass spectrometry (LC-MS) metabolomics was used study its role in intra- extracellular metabolites. A comparison ma...

We measured the erythrocyte levels of principal nucleotides (ATP, ADP, AMP, GTP, GDP, GMP, IMP), nucleosides (Ado, Guo, Ino) and Hyp with HPLC. Purine concentrations were determined in the erythrocytes of 36 type 1 and 40 type 2 diabetic patients. The increased dephosphorylation of adenine and guanine nucleotides, indicated by increased Ado, Ino, Guo and Hyp concentrations as the products of pu...

Amethopterin in toxic amounts, whether given in a single dose or in repeated daily doses, produced no observable inhibition of the nicotinamide-induced synthesis of diphosphopyridine nucleotide in mouse liver in vivo. Likewise, no inhibition was de tected of the incorporation of formate-C14 into the total liver acid-soluble adenine. However, administration of amethopterin sufficient to produce ...