Phospholipase D (PLD) plays an important role in plants, including responses to abiotic as well as biotic stresses. A survey of the rice (Oryza sativa) genome database indicated the presence of 17 PLD genes in the genome, among which OsPLDa1, OsPLDa5, and OsPLDb1 were highly expressed in most tissues studied. To examine the physiological function of PLD in rice, we made knockdown plants for eac...

Çeltik, insan beslenmesinde önemli besin kaynaklarından birisidir. Çeltik yanıklık hastalığına neden olan Pyricularia oryzae çeltik yetiştirilen alanlarda görülen en yıkıcı hastalıktır. Bu çalışmanın amacı Akdeniz Bölgesinde alanlarında karşı farklı ilaçlama ve ekim şeklinin hastalık şiddeti verim üzerine etkisinin araştırılmasıdır. Tarla denemeleri, 2011 2012 yıllarında sırasıyla Adana İli Yür...

The global call on the use of botanicals sequel to control of phytopathogenic microorganisms causing diseases of food crops most especially on rice has not been given attention in the tropics. A study was conducted to evaluate the antagonistic effect (in-vitro) of aqueous and ethanol extracts of Eucalyptus lameldulensis and Moringa oleifera leaves at different concentration (5, 10, 20, 40 and 1...

Introduction: Alpha-Momorcharin (α-MMC) is a member of the ribosome-inactivating protein (RIP) family that has been widely used as an antitumor, antiviral and antifungal agent. Methods: In this study, codons DNA encoding α-MMC were optimized for expression in E. coli cloned into pET-28a(+) vector. The was then expressed strain BL21 (DE3) purified by nickel affinity chromatography. Results: Unde...

Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe ory...

Phospholipase D (PLD) plays an important role in plants, including responses to abiotic as well as biotic stresses. A survey of the rice (Oryza sativa) genome database indicated the presence of 17 PLD genes in the genome, among which OsPLDalpha1, OsPLDalpha5, and OsPLDbeta1 were highly expressed in most tissues studied. To examine the physiological function of PLD in rice, we made knockdown pla...