The identification of transforming genes in human tumor cells has been made possible by DNA mediated gene transfer techniques. To date, it has been possible to show that most of these transforming genes are activated cellular analogues of the ras oncogene family. To better understand the relationship between these oncogenes and other human genes, we have determined their chromosomal localizatio...

The cytogenetic characteristics of osteosarcoma (OS) remain controversial. The establishment of a new human OS cell line may improve the characterization. We report the establishment of a new human osteosarcoma cell line, UTOS-1, from a typical osteoblastic OS of an 18-year-old man. Cultured UTOS-1 cells are spindle-shaped, and have been maintained in vitro for over 50 passages in more than 2 y...

A molecular cytogenetic analysis was performed on HS-RMS-2, a cell line established in this laboratory from a rare pleomorphic type of rhabdomyosarcoma. G-banding and multicolor-FISH analyses revealed that the cells have a complex chromosomal composition. Comparative genomic in situ hybridization (CGH) detected eight highly amplified regions at 1p36.1-p36.2, 1p31-p32, 1q21-q31, 8q12-q21, 8q24-q...

BACKGROUND Glioblastoma multiforme (GBM) displays multiple amplicons and homozygous deletions that involve relevant pathogenic genes and other genes whose role remains unknown. METHODOLOGY Single-nucleotide polymorphism (SNP)-arrays were used to determine the frequency of recurrent amplicons and homozygous deletions in GBM (n = 46), and to evaluate the impact of copy number alterations (CNA) ...

Translocations in hematologic disease of myeloid or lymphoid origin with breakpoints at chromosome band 12p13 frequently result in rearrangements of the Ets variant gene 6 (ETV6). As a consequence either the ETS DNA-binding domain or the Helix-Loop-Helix (HLH) oligomerization domain of ETV6 is fused to different partner genes. We show here that a t(9;12)(p24;p13) in a case of early pre-B acute ...

Introduction: The use of realistic simulation methodology is used in several learning scenarios, allowing students to participate directly the problematization situations that require immediate professional action. Objective: To develop, validate and a low cost simulator for cardiopulmonary resuscitation procedures infants. Methods: An experimental study carried out with undergraduate 1st year ...

the present study was conducted to investigate qualitative and quantitative aspects of irrigated wheat production in three major farming systems i.e. peasant, commercial and cooperative systems in kordestan province. accordingly, seven basic hypotheses were tested. the data were drawn from individual interviews with 360 farmers who planted wheat in 2004-2005. the sample was selected using multi...

This paper investigates the effects of inequality in the presence of voluntary risk-sharing. In any period, an agent’s resources are composed of his share of a secure endowment (wealth, land) and a random component (labor income). To be sure, the distribution of wealth does not affect the Pareto optimal payoff vectors but, by changing the autarchic utilities, affects the set of these payoff vec...

Salla disease (SD) is an autosomal recessive disorder in which free sialic acid (N-acetyl neuraminic acid) accumulates in lysosomes. A specific transport mechanism for acidic monosaccharides on the lysosomal membrane has recently been described, but the molecular deficiency causing SD is still unknown. We have previously mapped the SD gene to 6q14-q15 by means of genetic linkage analysis and re...