Sperm acrosomes contain hyaluronidase, the amount varying, respectively, from ram, rabbit, bull, human, boar, rat, and stallion sperm to rooster sperm that do not possess this enzyme. Hyaluronidase was partially purified from bull sperm acrosomal extracts by DEAE-cellulose chromatography. Bull sperm acrosomal hyaluronidase has an optimum pH of 3.75 and is not effected by freezing and thawing or...

The effects of holding diluted ram semen at 5 ◦C for up to 48 h prior to cryopreservation were investigated. Semen from six rams was collected by electro-ejaculation in the autumn and again from six different rams in the spring. The sperm concentration and motility were determined using spectrophotometry and computerized automated semen analysis, respectively. Samples were diluted at 23 ◦C to 4...

Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen sam...

Abstract The study was executed to investigate the different cooling periods (0, 24, and 48 hours) effects on plasma membrane integrity depending hypo osmotic swelling test (HOST), spermatozoa motility, acrosome integrity, live sperm, abnormalities, correlation among Host other rams sperm parameters This done at an animal farm in Al-Kut city (southern Baghdad city\ Iraq), from 5th January Febru...

The primary cause of sperm quality decline during the freeze-thaw pathway is peroxidation hazard caused by reactive oxygen species produced biological molecules sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed analyse effects ascorbic on motility, viability, abnormality plasma membrane integrity post-thawed S...

Seminal plasma is comprised of the extracellular sperm milieu from spermatogenesis in the testes, through to maturation in the epididymis, as well as secretions from the accessory sex glands. Consisting primarily of proteins, this substance has a multitude of functions in mammalian reproduction. Seminal plasma influences sperm physiology, success of semen freezing, migration of spermatozoa thro...

The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation tropical hair breeds. ejaculates were collected from 8 rams (4 Blackbelly and 4 Pelibuey), which mixed (pooled) by breed, diluted cryopreserved in three different extenders: Animal protein [Tris egg yolk (Triladyl®)], AndroMed® (lecithin soy bean) OPTIXcell® (liposome). Sperm analyses total (TM) pr...