I. The characteristics of sugar transport in the isolated rat uterus were studied using the non-metabolized glucose analogue 3-0-methyl-D-glucose. The effect of estrogen injection into ovariectomized rats on the transport rate was also determined. 2. Estrogen injection increased the initial rate of 3-0-methylglucose transport into uterine cells. The effect was evident at 2 h, and maximal at 4 h...

The present paper reports measurements on the electrolyte content of immature rat uteri before and after the administration of a single dose of estrogen. It has been shown previously that estrogen causes a prompt and striking increase in the weight and water content of rat uteri and that these changes are followed by a further gain in weight associated with an increase in mitotic activity and p...

A subclone of rat pituitary tumor cells, designated GH3/C14, was isolated from the parent population of GH3 pituitary cells and was estrogen-dependent for growth in vivo. GH3/C14 cells inoculated into host animals formed tumors in intact females, estrogen-treated ovariectomized females, and estrogen-treated males, but not in untreated intact or castrated males, or untreated ovariectomized femal...

WS-7528, produced by Streptomyces sp. No. 7528, was extracted from cultured broth, purified by solvent extraction followed by chromatography on silica gel and then isolated as pale yellow powder (C16H14O5, mp 95-98 degrees C). WS-7528 inhibited estrogen binding to its receptor protein in rat uterine cytosol. The IC50 value of WS-7528 for partially purified rat uterine cytosol receptor was 5.7 x...

BACKGROUND Flavonoids, a group of compounds mainly derived from vegetables and herbal medicines, chemically resemble estrogen and some have been used as estrogen substitutes. Kaempferol, a flavonol derived from the rhizome of Kaempferia galanga L., is a well-known phytoestrogen possessing osteogenic effects that is also found in a large number of plant foods.The herb K. galanga is a popular tra...

Male rat copulation is mediated by estrogen-sensitive neurons in the medial preoptic area (MPO) and medial amygdala (MEA); however, the mechanisms through which estradiol (E2) acts are not fully understood. We hypothesized that E2 acts through estrogen receptor α (ERα) in the MPO and MEA to promote male mating behavior. Antisense oligodeoxynucleotides (ASODN) complementary to ERα mRNA were bila...

INTRODUCTION: The ovariectomised (OVX) rat is currently the most popular animal model used for studies on estrogen deficiency-induced or postmenopausal osteoporosis. However, an issue with the OVX rat model is that there is no standard model. Throughout the literature, investigators have used animals that were ovariectomised at different ages, started their observations, treatments, etc. at var...

It is well known that the incidence of urinary stones is higher in men than women. Although it is believed that the lower incidence of urinary stones in women is due to a protective effect of estrogen, the mechanisms remain unclear. To clarify the relation between female sex hormones and stone matrix protein, we examined the interaction of estrogen receptor-α (ERα), estrogen receptor-related re...