Abstract Background The molecular mechanisms for chronic kidney disease (CKD) remain largely unknown and appear to be multifactorial. In the current study, we aimed study circulatory levels of circular ankyrin repeat domain 36 (circANKRD36) ANKRD36 in Egyptian patients with type 2 diabetes mellitus (T2DM) CKD explore their associations progression CKD. This cross-sectional controlled enrolled 6...

OBJECTIVE Molecular cloning and bioinformatics analysis of annexin A2 (ANXA2) gene in sika deer antler tip were conducted. The role of ANXA2 gene in the growth and development of the antler were analyzed initially. METHODS The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the ANXA2 gene from antler tip of sika deer (Cervus Nippon hortulorum) a...

INTRODUCTION Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) RNA in clinical samples have been extensively described. Routine commercial methods for each specific purpose (detection, quantification and genotyping) are also available, all of which are typically based on polymerase chain reaction (PCR) targeting the HCV 5' untranslated region (5'UTR). This st...

background and aims: accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (fmd). in present study, was evaluated real time reverse transcription-polymerase chain reaction (rrt-pcr) assay along with diagnostic routine methods for the detection of all seven serotypes of fmd virus (fmdv), namely o, c, a, sat1, 2, 3 and asia 1 in biological sample...

background: the aim of this study was to compare the effects of diode laser irradiation on wound healing in oral rat mucosa and also to measure the amount of inducible nitric oxide synthase (inos) and endothelial nos (enos) on oral wound healing. healing was assessed by histology and the amounts of enos and inos were measured by real‑time polymerase chain reaction (pcr). materials and methods: ...

Aim: Our aim in this study is to determine the C-reactive protein/Albumin ratio (CAR) values of computed tomography (CT) -positive COVID-19 patients and CT-negative who are Real-Time Polymerase Chain Reaction (RT-PCR) investigate relationship between protein / Albumin radiological images RT-PCR positive patients.
 Material Methods: 100 over age 18 were included our study. (CRP), albumin le...

 Background: Influenza A virus is the most virulent human pathogen and causes the most serious problem. Having epidemiological knowledge about this disease is important. The aim of this study was to determine the prevalence of influenza A/H3N2 virus infection in northern Iran from 2011 to 2013 using the real-time polymerase chain reaction (RT-PCR). Methods: In this cross-sectional study...