recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

background and objectives: brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against brucella is desirable. in this study, we evaluated the immune responses induced by a designed recombi...

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5o and 3o ends of SAG2, respectively, with...

visceral leishmaniasis (vl) is a zoonotic disease caused by leishmania species. dogs are considered to be the main reservoir of vl. a number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. however, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. a variety of serological tests ...

background: interleukin-27 is a heterodimeric cytokine belonging to il-12 and il-23 families, secreted  by antigen presenting cells( apcs ) .the il-27 is composed of 2 subunits: epstein-barr virus induced gene 3 (ebi3) and p28. il-27 is an anti-inflammatory cytokine which has an inhibitory effect on th17 population and suppress the il-17 expression. it is suggested that il-27 could be a potent ...

white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including iran. in the present study, a pair of primers was designed according to the sequence of vp 28 gene of wssv in the genbank. vp28 gene from an iranian wssv isolate (irvp28) was cloned, sequenced and expressed in escherichia coli bl21(de3) strain in order to produce vp28 protein...

Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from enceph...

This study was performed in order to evaluate the sero-epidemiological status of toxoplasmosis in pregnant Korean women. Among 5,175 sera and 750 amniotic fluid samples obtained from pregnant women, 41 serum samples (0.79%) and 10 (1.33%) amniotic fluid samples tested positive for IgG antibodies by ELISA. Fifty one cases showing a score more than 0.25 on ELISA were tested for PCR reaction again...