Lipase-displaying whole cells appear to be efficient biocatalysts because of their low preparation costs and simple recycling procedure. The combined utilization of Candida antarctica lipase B (CALB) and Rhizomucor miehei lipase (RML), separately displayed on Pichia pastoris whole cells, to produce biodiesel in co-solvent media was investigated. A response surface methodology incorporating a D-...

Mucormycosis is a disease caused by the fungi belonging to order mucorales which affects mainly immunocompromised patients. These are found in soil and decomposition of plants animals from sporangiospores released air then inhaled resulting infection based on host resistance. The cases increasing conditions with malnutrition, diabetes, steroid therapy acidosis. After aspergillosis candidiasis, ...

Shaoxing rice wine is a notable exemplar of Chinese wine. Its superior quality strongly correlated with the indigenous natural environment. The results indicated that Firmicutes (75%), Actinobacteria (15%), Proteobacteria (5%), and Bacteroidetes (3%) comprised prevailing bacterial groups. Among main genera, Lactobacillus was most abundant, accounting for 49.4%, followed by Lactococcus (11.9%), ...

In this study, Rhizomucor miehei ?-1,3-1,4-glucanase gene was expressed under the regulation of AOX1 promoter in Pichia pastoris expression system, and clone providing highest production level determined. The codon-optimized ligated into vector pPICZ?A transferred competent P. KM71H cells. Thirty transformants were selected from plates containing different concentrations zeocin cultured test tu...