Strategies to manage metabolic hydrogen ([H]) in the rumen should be considered when reducing ruminant methane (CH4) emissions. However, little is known about the use of dietary treatments to stimulate rumen microorganisms capable of capturing the [H] available when CH4 is inhibited in vivo. The effects of the phenolic compound phloroglucinol on CH4 production, [H] flows and subsequent response...

OBJECTIVE The aim of the study was to isolate gossypol-degrading bacteria and to assess its potential for gossypol degradation. METHODS Rumen liquid was collected from fistulated cows grazing the experimental pasture. Approximately 1 mL of the rumen liquid was spread onto basal medium plates containing 2 g/L gossypol as the only source of carbon and was then cultured at 39°C to isolate gossyp...

Background: Aflatoxins are fungal secondary metabolites negatively affecting ruminant performance; however, little information is available on their impact on rumen fermentation. Aims: This study aimed at determining the effects of different concentrations of aflatoxin B1 (AFB1) from Aspergillus flavus on in vitro gas production and ruminal fe...

Ruminants obtain nutrients from microbial fermentation of plant material, primarily in their rumen, a multilayered forestomach. How the different layers of the rumen wall respond to diet and influence microbial fermentation, and how these process are regulated, is not well understood. Gene expression correlation networks were constructed from full thickness rumen wall transcriptomes of 24 sheep...

greenhouse gases have been of serious global concern to environmentalists. enteric ruminal fermentation and manure are seen to be responsible for global warming. based on a better understanding of positive tan-nin effects on ruminant nutrition, the feeding value of browse trees and shrubs containing tannin, their roles on methanogenic rumen microbes in overcoming the production of enteric ferme...

This study was conducted to investigate effects of disodium fumarate (DF) on fermentation characteristics and microbial populations in the rumen of Hu sheep fed on high-forage diets. Two complementary feeding trials were conducted. In Trial 1, six Hu sheep fitted with ruminal cannulae were randomly allocated to a 2 × 2 cross-over design involving dietary treatments of either 0 or 20 g DF daily....

Ruminant-derived methane (CH4), a potent greenhouse gas, is a consequence of microbial fermentation in the digestive tract of livestock. Development of mitigation strategies to reduce CH4 emissions from farmed animals is currently the subject of both scientific and environmental interest. Methanogens are the sole producers of ruminant CH4, and therefore CH4 abatement strategies can either targe...

The rumen microbiota enable ruminants to degrade complex ligno-cellulosic compounds to produce high quality protein for human consumption. However, enteric fermentation by domestic ruminants generates negative by-products: greenhouse gases (methane) and environmental nitrogen pollution. The current lack of cultured isolates representative of the totality of rumen microbial species creates an in...

Two experiments using six rumen-fistulated Holstein steers in 3 X 3 Latin square designs were conducted to test the effects of urea addition to corn silage, and grain addition to haylage, on feed intake, digestibility of nutrients and rumen fermentation of complete diets fed once daily. Addition of .34 and .68% urea to corn silage increased dietary crude protein from 8.1 to 11.3 and 13.3%, and ...