Findings are presented from a prospective cohort study of timing of primary tooth emergence and timing of oral colonization of Streptococcus mutans (S. mutans) in Australian twins. The paper focuses on differences in colonization timing in genetically identical monozygotic (MZ) twins. Timing of tooth emergence was based on parental report. Colonization timing of S. mutans were established by pl...

Dental caries is one of the most common prevailing disease in humans and a significant public health problem worldwide. Mutans streptococci are a group of oral bacteria, among this group Streptococcus mutans and Streptococcus sobrinus are frequently associated with dental caries. The aim of the present study was (i) To investigate the reliability of morphological, biochemical and multiplex PCR ...

Article history: Received on: 11/05/2015 Revised on: 29/05/2015 Accepted on: 09/06/2015 Available online: 27/06/2015 Dental caries is one of the oldest disease in the world and its causative agent is mutans streptococci (MS). Among MS, Streptococcus mutans and Streptococcus sobrinus are implicated in caries active subjects. The objective of this study was to identify and determine the antibiogr...

Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mutans among children attending one nursery suggests horizontal transmission.

Homeostasis of oral microbiota can be maintained through microbial interactions. Previous studies showed that Streptococcus oligofermentans, a non-mutans streptococci frequently isolated from caries-free subjects, inhibited the cariogenic Streptococcus mutans by the production of hydrogen peroxide (HP). Since pH is a critical factor in caries formation, we aimed to study the influence of pH on ...

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Culture methods traditionally have been used to isolate and identify bacterial species in clinical specimens. Culture methods are difficult when the specimen is as bacteriologically complex as dental plaque1 and when the organism in question, such as Streptococcus mutans, is present in modest numbers. The fluorescent antibody test (FAT) has been suggested as a potentially simpler method of dete...

Genomic libraries from Streptococcus mutans OMZ175 were constructed in bacteriophage vectors. DNA fragments 1 to 2 kilobases in length were cloned in expression vector lambda gt11. S. mutans DNA fragments 15 to 20 kilobases in length were inserted in the BamHI site of phage EMBL3. Rabbit antiserum raised against an S. mutans saliva-interacting protein with a molecular weight of 74,000, designat...

Streptococcus mutans, the primary etiological agent of human dental caries and an obligate biofilm-forming bacterium, has developed a variety of mechanisms to colonize the tooth surface. Oral transmission of S. mutans through contact between mother and child is thought to be one of the risks of developing dental caries. In this study, I surveyed oral transmission frequency of S. mutans from mot...

The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-beta-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. T...