The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture condit...

Carbonyl carotenoids are important constituents of the antenna complexes of marine organisms. These carotenoids possess an excited state with a charge-transfer character (intramolecular charge transfer state, ICT), but many details of the carotenoid to chlorophyll energy transfer mechanisms are as yet poorly understood. Here, we employ femtosecond transient absorption spectroscopy to study ener...

The production of inoculum is one of the hindrances in the large scale application of arbuscular mycorrhizal fungi (AMF). The objective of this work was to evaluate the effect of nutrient solutions with or without TrisHCl buffer, on sporulation of AMF. The experiment was carried out in a greenhouse, using a substrate with sand and vermiculite (1:1 v/v). Fifty spores of Gigaspora margarita, Scut...

Recent studies suggest that 1) electrically induced fibrillation and defibrillation involve prolongation of refractoriness by the shock in addition to stimulation and 2) biphasic waveforms are more efficient for defibrillation than are comparable monophasic waveforms. The purpose of this study was to compare prolongation of action potential duration at 50% repolarization by monophasic and bipha...

The brain representation of the body, called the body schema, is susceptible to plasticity. For instance, subjects experiencing a rubber hand illusion develop a sense of ownership of a mannequin hand when they view it being touched while tactile stimuli are simultaneously applied to their own hand. Here, the cortical basis of such an embodiment was investigated through concurrent recordings fro...

The toxicity of pertussis toxin is mediated by the ADP-ribosyltransferase activity of subunit S1. To understand the structure-function relationship of subunit S1 and guide the construction of nontoxic molecules suitable for vaccines, we constructed and expressed in Escherichia coli a series of amino-terminal and carboxyl-terminal deletion mutants as well as a number of molecules containing amin...

In vitro experiments to study interaction of the mutagenic flavonoid quercetin with DNA are described. Calf thymus DNA treated with quercetin for various time periods was subjected to S1 nuclease hydrolysis. Thermal melting profiles of treated DNA were also determined using S1 nuclease. The rate of DNA hydrolyzed after 1 hr of pretreatment with quercetin was found to be only about 50% of that i...

Secondary flow is a prominent feature of channel bends; it alters the streamwise velocity and bed shear stress distributions. Experiments were conducted to investigate complex pattern secondary in narrow sharp open-channel bend underlying mechanism generation multiple circulation cells. Compared with moderate bends, bends are characteristic cells from 90° section. In addition curvature-induced ...

This study aimed to evaluate greenhouse gas (GHG) emissions from conventional cultivation (S1) of seedless lime (SL) fruit in Hau Giang province, the Mekong Delta region Vietnam. We adjusted scenarios by replacing 25% and 50% nitrogen chemical fertilizer with respective amounts N-based organic (S2 S3). Face-to-face interviews were conducted collect primary data. Life cycle assessment (LCA) meth...