Fluorescence spectroscopy was examined as a potential technique for identification and classification of bacterial pathogens. Colonies of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae on agar plates were measured directly using a laboratory spectrofluorimeter coupled with optical fiber. Steady state fluorescence spectra were collected following excit...

Methanol and aqueous extracts from fi ve plant species, used in traditional medicine in Guatemala for the treatment of microbial infections, were tested in vitro for their ability to scavenge DPPH, OH(.) and O(2) (-) radicals and to inhibit lipoperoxidation (LPO) in order to establish a relationship between their antioxidant activities and their effects against infectious agents. Acalypha guate...

The effects of the methanol extract of the stem bark of Prosopis africana (Guill., Perrott. and Rich.) Taubert (Fabaceae) on bleeding/clotting and coagulation time, excision and dead space wounds were studied in rats. Also, the extract was subjected to antibacterial, and acute toxicity and lethality (LD(50)) tests. The extract significantly (P<0.05) reduced bleeding/clotting and coagulation tim...

A series of 1,3,5-triazine derivatives that contain 4-amino-2-trifluoromethyl-benzonitrile, 8-hydroxyquinoline, and different piperazines as substituents at the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. The chemical structures of the compounds were elucidated with the aid of IR, 1H NMR and 13C NMR spectroscopy, and elemental analysis. ...

The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride ion channel that also serves as a receptor for entry of Pseudomonas aeruginosa and Salmonella enterica serovar Typhi into epithelial cells. To evaluate heterogeneity in CFTR protein expression in cultured cells and the effect of heterogeneity on internalization of different P. aeruginosa and serovar Typhi strains, we u...

A systematic approach was taken to isolate and characterize the antimicrobial peptide(s) from the crude aqueous extract, solubilized ammonium sulphate precipitates and purified gel filtration chromatographic fractions of seed/pod of Pisum sativum L. (garden pea). Their antibacterial activity was investigated against a number of bacteria: Micrococcus luteus, Staphylococcus aureus, Staphylococcus...

To overcome the burden of antimicrobial resistance, greater attention has been paid to activity screening from natural products. One such products used for therapeutic purposes is Alum. Besides, Ayurvedic Bhasma formulations have treatment various ailments thousands years all over world and widely in India. Hence, present vitro study, Alum as well its Bhasma- Sphatika was evaluated against tota...

The present study has been carried out to investigate the antibacterial activity of molluscan gastropod species Lambis lambis and to find out the possible bioactive compounds present in the body tissue extract of the experimental animal. The antibacterial activity of extracts of body tissue of L.lambis against 8 bacterial pathogens viz., Bacillus subtilis, Escherichia coli, Salmonella typhi, Kl...

The aim of the present study was to investigate the antimicrobial and preliminary phytochemical properties of Lantana indica Roxb. The aqueous and organic solvent (ethyl acetate and methanol) extracts from the leaves of Lantana indica (Verbenaceae) were tested against Staphylococcus aureus, Bacillus subtilis, Steptococcus pyrogens, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Pseu...

Clinically, antimicrobial susceptibility testing results provide guidance in the choice of agents patient care. The accuracy from can be affected by multiple factors including media, discs or preparations, inoculum’s size, plate reading and incubation conditions. Misleading test leads to indiscriminate irrational use antibiotics have impacted grossly global challenge resistance. objectives this...