نتایج جستجو برای: salting
تعداد نتایج: 1231 فیلتر نتایج به سال:
An amphiphilic aryl-ether AB2-type dendron(1)bearing oligo ethylene glycol(OEG)chains exhibited lower critical solution temperature behavior in aqueous solutions. In the presence of salting-out salts(Na2SO4, NaCl, and NaBr), cloud points 1 decreased linearly with increasing salt concentration. The slope linear decrease changed anion type order, SO42- > Cl- Br-. salting-in salts(NaSCN NaI), incr...
Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation s...
Received 04/07/2010 Accepted 28/02/2012 (004909) 1 Chemistry Department, State University of Londrina – UEL, PO Box 6001, CEP 86051-990, Londrina, PR, Brazil, e-mail: [email protected] 2 Food Science Department, State University of Londrina – UEL, PO Box 6001,CEP 86051-990, Londrina, PR, Brazil 3 Food Engineering Department, Federal University of Technology – UTFPR, PO Box 271, CEP 87301-006, Cam...
A polysaccharide depolymerase was puritied 1,688-fold from crude lysates produced by the propagation of a specitic bacteriophage in Pseudomonas aeruginosa. Conventional methods of protein fractionation were employed, namely, salting out with ammonium sulfate, Sephadex gel filtration, and high speed centrifugation. The purified depolymerase behaves as a single entity when tested by electrophores...
A polysaccharide depolymerase was puritied 1,688-fold from crude lysates produced by the propagation of a specitic bacteriophage in Pseudomonas aeruginosa. Conventional methods of protein fractionation were employed, namely, salting out with ammonium sulfate, Sephadex gel filtration, and high speed centrifugation. The purified depolymerase behaves as a single entity when tested by electrophores...
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