The tectorial membrane is an extracellular matrix lying over the apical surface of the auditory epithelium. Immunofluorescence studies have suggested that some proteins of the avian tectorial membrane, the tectorins, may be unique to the inner ear (Killick, R., C. Malenczak, and G. P. Richardson. 1992. Hearing Res. 64:21-38). The cDNA and deduced amino acid sequences for chick B-tectorin are pr...

Tetracenomycin (Tcm) F2 cyclase, which catalyzes the cyclization of the anthrone Tcm F2 to the naphthacenone Tcm F1 in the biosynthesis of the anthracycline antibiotic Tcm C in Streptomyces glaucescens, has been purified to homogeneity and characterized. The N-terminal sequence of the enzyme establishes that it is encoded by the tcml gene, whose deduced product has a molecular weight of 12 728....

immune sera, polyclonal rabbit antibodies to human IL-1 f3, or anti-p20 ICE and protein A-agarose. After extensive washes, the adsorbed proteins were eluted by boiling the beads in SDS-PAGE sample buffer and analyzed by SDS-PAGE and autoradiography. 13. The ICE homologs and p35 were expressed in E coli under the control of the X pL promoter. ICH-1 (7) and CPP32o (8) contained a polyhistidine li...

Detection of membrane-bound proteins by immunoblot (Western blot) is by far the most commonly used procedure for detection or quantitation of an antigen (11). However, since this technique requires analysis of the antigen on a denaturing gel using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the antigen generally loses its native conformation (7). Although resolution ca...

Semiconductor nanocrystals have been shown to possess unique optical, electrical, and optoelectronic properties for a wide range of applications. In particular, the exploitation of semiconductor nanocrystals, often referred to as quantum dots, in biological applications has increased dramatically because of their unique spectral properties, which enable simultaneous multiplex labeling and detec...

The tectorial membrane is an extracellular matrix lying over the apical surface of the auditory epithelium. Immunofluorescence studies have suggested that some proteins of the avian tectorial membrane, the tectorins, may be unique to the inner ear (Killick, R., C. Malenczak, and G. P. Richardson. 1992. Hearing Res. 64:21-38). The cDNA and deduced amino acid sequences for chick B-tectorin are pr...

Expression of foreign proteins in E. coli is frequently stymied by the formation of insoluble inclusion bodies. To recover the insoluble protein, the inclusion body is usually solubilized with a denaturing reagent such as urea and guanidine HCl, followed by dialysis to renature the target protein. Nondetergent sulfobetaines (NDSB) are a family of zwitterionic compounds carrying a sulfobetaine h...

References and Notes 1. J. A. Bluestone, J. Immunol. 158, 1989 (1997); A. I. Sperling and J. A. Bluestone, Immunol. Rev. 153, 155 (1996). 2. C. R. Calvo, D. Amsen, A. M. Kruisbeek, J. Exp. Med. 186, 1645 (1997), P. S. Linsley et al., Immunity 4, 535 (1996); M. Griffin and J. A. Bluestone, unpublished observations. 3. W. Zhang et al., Cell 92, 83 (1998). 4. K.-M. Lee et al., unpublished observat...

Localization of the Ca + Mgt+-ATPase of the sarcoplasmic reticulum in rat papillary muscle was determined by indirect immunofluorescence and immunoferritin labeling of cryostat and ultracryotomy sections, respectively . The Ca + Mgt+ -ATPase was found to be rather uniformly distributed in the free sarcoplasmic reticulum membrane but to be absent from both peripheral and interior junctional sarc...

Intact proteins comprise a major component of organic carbon and nitrogen produced globally and are likely an important fraction of organic matter in sediments and soils. Extracting the protein component from sediments and soils for mass spectral characterization and identification represents a substantial challenge given the range of products and functionalities present in the complex matrix. ...