Three sensitive and specific assays, the lateral flow assay (LFA), polymerase chain reaction assay (PCR) and reversed passive latex agglutination assay (RPLA), were selected for detection of staphylococcal enterotoxin B (SEB) from 77 clinical Staphylococcus aureus strains isolated from humans. Analytical results revealed that the LFA has almost the same detection sensitivity as that of PCR and ...

Staphylococcal superantigens (SAG) are implicated in the inflammation of atopic dermatitis. As SAG mediated diseases may be modified by specific antibodies, the antibody response to SAG in atopic dermatitis was investigated. Immunoglobulin (Ig) G to staphylococcal enterotoxin A (SEA), staphylococcal enterotoxin B (SEB), and toxic shock syndrome toxin 1 (TSST-1) were measured by sandwich enzyme ...

Staphylococcal enterotoxin B (SEB) is both a superantigen and toxin. As a superantigen, SEB can bind to major histocompatibility complex (MHC) class II molecules to form a ligand for ot/B T cell receptors bearing particular VB elements. As a toxin, SEB causes rapid weight loss in mice sometimes leading to death. We show here that both of these functions map to the NH2-terminal portion of the to...

This article introduces the Service Experience Blueprint (SEB), a multidisciplinary method for designing multiinterface service experiences, and illustrates its application with two case examples of the redesign of the service experiences of a multichannel bank. The SEB method starts by studying the customer service experience to understand customer experience requirements for different service...

We screened a panel of monoclonal antibodies against selected macrophage cell surface molecules for their ability to inhibit enterotoxin binding to major histocompatibility complex class II-negative C2D (H-2b) macrophages. Two monoclonal antibodies, HB36 and TIB126, that are specific for the alpha 2 domain of major histocompatibility complex class I, blocked staphylococcal enterotoxins A and B ...

In Palaeozoic rocks of South-eastern Bosnia (SEB) there are numerous, but very small vein and replacement barite ore deposits containing up to 10% Pb-Zn-Fe-Cu sulphides. Their geochemical characteristics are compared with large barite monosulphide (Hg-tetrahedrite) ore deposits in Mid-Bosnian Schist Mountains (MBSM). The 87Sr/86Sr isotope ratios in the barites of both areas are very similar, (0...

Abstract Background Cognitive ability and socioeconomic background (SEB) have been previously identified as determinants of achieved level education. According to a “discrimination hypothesis”, higher cognitive is required from those with lower SEB in order achieve the same education SEB. Support for this hypothesis has claimed observation positive association between when adjusting ability. We...

Single event burnout (SEB) is a great threat to gallium nitride (GaN) power devices for aerospace applications. This paper dedicated the investigation of SEB mechanism in GaN device using femtosecond pulsed laser. In test, commercial p-GaN was triggered by focused laser beam with wavelength 620 nm, and irradiation-sensitive area identified. We observed that damage modes were consistent results ...

Single-domain antibodies (sdAbs), derived from the heavy chain only antibodies found in camelids such as llamas have the potential to provide rugged detection reagents with high affinities, and the ability to refold after denaturation. We have isolated and characterized sdAbs specific to staphylococcal enterotoxin B (SEB) which bind to two distinct epitopes and are able to function in a sandwic...

It has been well established that bacterial superantigens lead to the induction and aggravation of chronic inflammatory skin diseases. We investigated the clinical significance of serum specific immunoglobulin E (lgE) to the staphylococcal superantigens staphylococcal enterotoxin A (SEA), staphylococcal enterotoxin B (SEB), and toxic shock syndrome toxin (TSST)-1 in patients with chronic urtica...