The continuous emergence of new variants concern for SARS-CoV-2 has created a challenge existing therapies. To address this, we developed series single-stranded DNA aptamers that not only bind specifically to the trimer S protein but also block interaction between and ACE2 receptors. systematic evolution ligands by exponential enrichment (SELEX) was performed select protein. ELISA-based assay f...

It has been 20 years since aptamer and SELEX (systematic evolution of ligands by exponential enrichment) were described independently by Andrew Ellington and Larry Gold. Based on the great advantages of aptamers, there have been numerous isolated aptamers for various targets that have actively been applied as therapeutic and analytical tools. Over 2,000 papers related to aptamers or SELEX have ...

Herpes simplex virus 1 (HSV-1) protein ICP27, an important regulator for viral gene expression, directly recognizes and exports viral RNA through an N-terminal RGG box RNA binding motif, which is necessary and sufficient for RNA binding. An ICP27 N-terminal peptide, including the RGG box RNA binding motif, was expressed and its binding specificity was analyzed using EMSA and SELEX. DNA oligonuc...