OBJECTIVE The main objective of this study was to assess semen characteristics of patients with testicular cancer before cryopreservation and after thawing, to evaluate the consequences of this technique on sperm quality in patients with testicular cancer. METHODS Five hundred eighty-nine samples from 543 patients with testicular cancer were cryopreserved between 1995 and 2015, one aliquot pe...

The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4°C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. Ho...

Abstract The European honey bee Apis mellifera is the main pollinator for most crops used human consumption. However, a number of diseases, parasites, pesticides and other factors that generally result in widely described colony collapse disorder weakens colonies. In order to maintain existing germ lines facilitate creation new disease-resistant lines, it necessary ensure consistent breeding wo...

This study assessed the effects of common extenders and diluents on the spermatozoa of African catfish (Clarias gariepinus), with the intent of obtaining a semen preservation protocol that can serve as a means of making fingerlings available to fish farmers all year round. Semen samples (milt) were pooled from mature broodstock males and pre-extension qualities were evaluated. Egg yolk, tomato ...

The recombinant protein of heterologous proteins in Escherichia coli strains K12 has various and different systems tested demands a detailed insight into the multiple factors affecting encoded protein. One crucial is acceptable quality DNA copies inserted inside bacteria. Firstly, amplification procedure needed to be performed well; thus, designing primer selecting optimum annealing temperature...