Deformability is an effective property that can be used in the separation of colloidal particles and cells. In this study, a microfluidic device is proposed and tested numerically for the sorting of deformable particles of various degrees. The separation process is numerically investigated by a direct numerical simulation of the fluid–particle–electric field interactions with an arbitrary Lagra...

Protocadherins have homophilic adhesion properties and mediate selective cell-cell adhesion and cell sorting. Knockdown of paraxial protocadherin (PAPC) function in the Xenopus embryo impairs tissue separation, a process that regulates separation of cells of ectodermal and mesodermal origin during gastrulation. We show that PAPC can modulate the activity of the Rho GTPase and c-jun N-terminal k...

this survey was done for constructing an egg sorting machine which could sort white eggs from fertilized eggs. egg sorting machine was constructed based on physiological properties of trout egg. fertilized eggs have much more elasticity than white or non-hatched eggs. so the hatched eggs will fly back if were hit to a solid surface. the machine divided to 5 parts including mechanical, electrica...

Cytokinesis and cell separation are critical events in the cell cycle. We show that Endosomal Sorting Complex Required for Transport (ESCRT) genes are required for cell separation in Schizosaccharomyces pombe. We identify genetic interactions between ESCRT proteins and polo and aurora kinases and Cdc14 phosphatase that manifest as impaired growth and exacerbated defects in septation, suggesting...

Isolation of phenotypically-pure cell subpopulations from heterogeneous cell mixtures such as blood is a difficult yet fundamentally important task. Current techniques such as fluorescent activated cell sorting (FACS) and magnetic-activated cell sorting (MACS) require pre-incubation with antibodies which lead to processing times of at least 15-60 min. In this study, we explored the use of antib...

Microfluidic continuous cell separation based on hydrodynamic interaction in a microfluidic channel has attracted attention because of its robustness, high throughput and cell viability. This paper systematically gives an overview on recent advances in hydrodynamic particle and cell separation in microfluidic devices. It presents the basic ideas and fluid mechanics for the hydrodynamic interact...

Sample preparation is often the most tedious and demanding step in an assay, but it also plays an essential role in determining the quality of results. As biological questions and analytical methods become increasingly sophisticated, there is a rapidly growing need for systems that can reliably and reproducibly separate cells and particles with high purity, throughput and recovery. Microfluidic...

The advent of microfluidic technology for genetic analysis has begun to impact forensic science. Recent advances in microfluidic separation of short-tandem-repeat (STR) fragments has provided unprecedented potential for improving speed and efficiency of DNA typing. In addition, the analytical processes associated with sample preparation--which include cell sorting, DNA extraction, DNA quantitat...

Separation/isolation of subcellular species, such as mitochondria, lysosomes, peroxisomes, Golgi apparatus, and others, from cells is important for gaining an understanding of the cellular functions performed by specific organelles. This study introduces a high speed, semipreparative scale, biocompatible size sorting method for the isolation of subcellular organelle species from homogenate mixt...

Source separation is a common method for dealing with the increasing problem of Municipal Solid Waste (MSW) in society. The citizens are then responsible for separating waste fractions produced in their home. If the consumers fail to sort the waste according to the source separation scheme, it will lead to an ineffective system. The purpose of this paper is to analyze the environmental, economi...