A new metabolite was isolated from the culture filtrate of a deacetylcephalosporin C-producing mutant, derived from Cephalosporium acremonium ATCC 14553, by means of adsorption on activated carbon, column chromatography on DEAE-Sephadex A-25 and gel filtration through a Sephadex G-10 column. The compound was identified as D-5-amino-5-carboxyvaleramido-(5-formyl-4-carboxy-2H, 3H, 6H-tetrahydro-1...

Riboflavin carrier (or binding) protein (RCP) was isolated from the Emu egg yolk (Dromaius novaehollandia) for the first time. In the present study an attempt has been made to isolate and purify the RCP in two steps, DEAE Sephadex A-50 ion exchange chromatography and the final purification was achieved on sephadex G-100.The purity of the protein was judged on cylindrical and slab gel electropho...

Cathepsin inhibitor has been purified to homogeneity from catfish muscle (Pangasius sp.). The purification was carried out through ammonium sulphate precipitation, followed by ion exchange chromatography using DEAE-Sephadex A-75, followed by gel filtration on Sephadex G-100. Throughout the purification procedure, cathepsin inhibitory activity was determined against hemoglobin. A single band of ...

1. Columns of Sephadex G-25 have been used for the preliminary fractionation of tryptic digests of casein. 2. Fractions from Sephadex columns have been examined for their ability to stimulate the growth of a strain of Streptococcus equi8imili8. Maximum stimulatory activity appeared to be localized in a fairly narrow region of the total peptide material. 3. Further fractionation of peptides deri...

The major peak of radioactivity from liver extracts appears to behave as a folate polyglutamate on Sephadex G-15, but its behaviour on DEAE cellulose is not consistent with a folate polyglutamate. The second, smaller peak behaved as a monoglutamate on both Sephadex G-15 and DEAE-cellulose, but could not be matched with any authentic folate monoglutamate. The present results fail to provide evid...

A thiamine-binding protein was purified from the extract of rice bran acetone powder by conventional procedures of acid precipitation, a series of column chromatography on DEAE-Sephadex A-50 and DEAE-cellulose, and gel filtration of Sephadex G-200. The purified thiamine-binding protein was nearly homogeneous as judged by disc gel electrophoresis and the molecular weight was estimated to be 94,0...

A serine collagenolytic protease was purified from the internal organs of filefish, Novoden modestrus, by ammonium sulfate, ion-exchange chromatography on a DEAE-Sephadex A-50, ion-exchange rechromatography on a DEAE-Sephadex A-50, and gel filtration on a Sephadex G- 150 column. The molecular mass of the filefish serine collagenase was estimated to be 27.0 kDa by gel filtration and SDS-PAGE. Th...

Hog thyroid tautomerase (EC 5.3.2.1, phenylpyruvate keto-enol isomerase), an enzyme which is believed to play a role in the biosynthesis of thyroxine, has been purified over lOOO-fold. The purification procedure involves a heat step at 70”, gel f&ration on Sephadex G-100, ion exchange chromatography on carboxymethyl Sephadex C-50, and, finally, gel titration on Sephadex G-50. The enzyme is homo...

Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was...

Preferential eosinophil chemotactic activity exhibiting a molecular weight comparable to that released from sensitized human lung fragments challenged with specific antigen and designated eosinophil chemotactic factor of anaphylaxis has been isolated from extracts of human lung fragments by sequential purification on Sephadex G-25, Dowex-1, Sephadex G-10, and paper chromatography. Two eosinophi...