The epidemiology of Pseudomonas aeruginosa infection was studied in Siriraj Hospital. During April 1989-June 1990, P. aeruginosa 436 strains were isolated from clinical specimens of 260 patients, ie blood (19 strains), pus (192 strains), sputum (159 strains) and urine (66 strains). By using a combination of serogroups and pyocin types as epidemiological markers, it was found that there were 10 ...

the clinical features of salmonellosis and prevalence of salmonella serogroups in calves were studied on one hundred and thirty two suspected calves in dairy farms around tehran. clinical examinations were carried out and the faecal or tissue (if calve was euthanized or dead) samples were collected for bacterial culture. the results were analyzed with chi-square statistical test. salmonella spp...

ELISA and antibody adsorption tests were applied to determine the minimal somatic antigen constitution of 243 strains of Bradyrhizobium sp. (Arachis) using 12 antisera. The 243 indigenous bradyrhizobial isolates were from 15 sites in four regions of Thailand. A total of 29 serogroups were identified. Most (80%) of the isolates tested had at least one heat-stable antigen in common with strain 28...

Neisseria meningitidis may cause invasive disease (meningitis and sepsis), leading to considerable disease burden and mortality. However, effective vaccines are available against most pathogenic serogroups. Large-scale vaccination campaigns with the MCC vaccine conducted in UK and with MenAfriVac in the Sahel have clearly demonstrated the direct and indirect effect of immunization programmes on...

What is meningococcal disease? Meningococcal disease is caused by the bacterium Neisseria meningitidis. The bacteria are transmitted from person to person through nasopharyngeal secretions. Close and prolonged contact is usually required for transmission. Most people carry the bacteria asymptomatically in the nose and throat. Disease occurs very rarely when bacteria invade the bloodstream. Meni...

Our Department of General Microbiology created a wide collection of P. penneri isolates and classified most of them into 19 different O-serogroups. This work describes the classification of 12 remaining P. penneri strains. The lipopolysaccharides extracted from P. penneri strains were tested in an enzyme-linked immunosorbent assay (ELISA) with selected O-antisera against Proteus sp. strains. Ho...

A semiautomated method for the determination of five serotypes and three serogroups in Streptococcus pneumoniae was developed. Primers specific for serotypes 1, 3, 14, 19F, and 23F and serogroups 6, 19, and 23 were combined in three multiplex PCRs. Products were separated by capillary electrophoresis with a 7-min run time, and a serotype or serogroup was assigned on the basis of fragment size. ...

Legionella pneumophila, the causative organism of Legionnaires' disease, is pathogenic for free living, ubiquitous, freshwater, and soil amoebae of the genera Acanthamoeba and Naegleria. Some species support the growth of strains from serogroups 1 to 6, others only strains from certain serogroups. Initial studies with seeded material indicate that amoebal enrichment could be utilised for the is...

Typhoidal and para-typhoidal Salmonella are major causes of bacteraemia in resource-limited countries. Diagnostic alternatives to laborious resource-demanding serotyping essential. Fourier transform infrared spectroscopy (FTIRS) is a rapidly developing simple bacterial typing technology. In this study, we assessed the discriminatory power FTIRS-based IR Biotyper (Bruker Daltonik GmbH, Bremen, G...