We studied the development of light scattering in the reaction between anti-apolipoprotein B and apolipoprotein B in intact very-low-density lipoproteins (I) and low-density lipoproteins (II) as well as in lipoproteins treated with lipases, and found considerable differences in the kinetics of the immunoreaction for the two lipoprotein classes. Pre-incubation with triglyceride lipase and choles...

Three major classes of lipoproteins (VLDL, d <1.006; LDL, d 1.006-1.040; HDL, d 1.063-1.21) were isolated by ultracentrifugal flotation from the serum of normal male Sprague-Dawley rats. Their physical, chemical, and immunological properties were analyzed and compared with those of their water-soluble, essentially lipid-free derivatives. Studies were also carried out on the d > 1.21 fractions. ...

After the administration of ccl4 to male rats, liver triglycerides began to increase after a lag period of about 1 hr; the level of serum triglycerides fell sharply during the first 30 min of intoxication. Three classes of serum lipoproteins were isolated by flotation in the ultracentrifuge and their concentrations and chemical compositions were determined. Within 4 hr of the administration of ...

Psoriasis is an epidermal disease characterized by chronic relapsing papillon squamous erythematous plaques which may develop in the skin of elbows, knees and extensor surfaces. The cause of psoriasis is unknown. The primary etiologic factor may be related to excessive uncontrolled synthesis is resulting from changes in (1) the function of growth factors ( EGF),(2) presence of proto- oncogenes,...

Dogs fed a diet containing butter, cholesterol, and thiouracil develop severe hypercholesterolemia. Both a p and 8-lipoprotein cholesterol and phospholipid increase whereas these lipids in the a, fraction appear to decrease. Comparison of the dextran sulfate-precipitable lipoproteins with lipoprotein fractions obtained by ultracentrifugation a t densities 1.019, 1.063, and 1.21 and with paper e...

The effect of dietary cholesterol on the lithogenesity of bile in female hamsters was investigated. Biliary and serum lipids and serum lipoproteins of the hamsters fed with the normal diet supplemented with 1% cholesterol for 10, 20 and 30 days were analyzed. The synthesis rate of phospholipids and triglycerides were determined with (l-14C) palmitate incorporated into these lipids in the livers...