We have examined the effect of the O-linked glycan (OLG) structures of VWF on its interaction with the platelet receptor glycoprotein Ib . The 10 OLGs were mutated individually and as clusters (Clus) on either and both sides of the A1 domain: Clus1 (N-terminal side), Clus2 (C-terminal side), and double cluster (DC), in both full-length-VWF and in a VWF construct spanning D to A3 domains. Mutati...

Treatment of proximal tibial defects is important to the survival of tibial prosthesis after total knee replacement. The objective of this finite element study was to determine a better understanding of the stresses produced by different treatment options for moderate uncontained type-2 defects. Methods analysed were the use of metal wedges, metal blocks, cement wedges, and cement blocks for th...

Unsteady computational simulations of a multi-element, high-lift configuration are performed. Emphasis is placed on accurate spatio-temporal resolution of the free shear layer in the slat-cove region. The excessive dissipative effects of the turbulence model, so prevalent in previous simulations, are circumvented by switching off the turbulence-production term in the slat cove region. The justi...

Bacteria can form biofilm streamers in microfluidic channels with various geometries. Experiments show that the streamer geometry, such as its shape or thickness, depends on the fluid velocity and the geometry and curvature of the microfluidic channel. In the paper, a mechanical analysis of the flow field is made in different channels, which shows that the secondary flow in the channel is the r...

Simulation of scour-pit formations under the bridge decks and around the bridge piers, due to sediment suspension and transport caused by flooding and pressure flow conditions, is of significant interest nowadays to computational fluid dynamics (CFD) and hydraulic researchers. From CFD perspective, analysis of bridge under various flooding conditions and impact of the drag and lift forces on it...

We compute rates of sediment removal from the Columbia River, USA plume near field using a control volume technique. By using data from repeated transect passes along the plume axis, we account for variations in plume width, depth, and velocity, and we construct a framework for determining the dominant terms in the complete sediment balance. The contribution due to turbulent sediment flux is es...

The purpose of this in vitro study was to evaluate the shear bond strength of glass ionomer sealer to dentine exposed to common endodontic irrigants in both well and poorly prepared dentine surface. In the methodology four groups of elephant dentine sample were used. Each group was well cleaned and prepared with different irrigants for the effective removal of smear layer. One group was treated...

The P2X1 receptor is a fast ATP-gated cation channel expressed in blood platelets, where its role has been difficult to assess due to its rapid desensitization and the lack of pharmacological tools. In this paper, we have used P2X1-/- and wild-type mouse platelets, treated with apyrase to prevent desensitization, to demonstrate the function of P2X1 in the response to thrombogenic stimuli. In vi...

The load-displacement behavior of 35 fresh adult cervical spine motion segments was measured in compression, shear, flexion, extension, lateral bending and axial torsion tests. Motion segments were tested both intact and with posterior elements removed. Applied forces ranged to 73.6 N in compression and to 39 N in shear, while applied moments ranged to 2.16 Nm. For each mode of loading, princip...

Endothelial function is noninvasively assessed by measuring nitric oxide-dependent increase in radial artery diameter accompanying the elevation in shear stress induced by increasing blood flow through a short-lasting ischemia of the hand. However, shear stress also depends on blood viscosity, whose changes might thus affect nitric oxide increase in a manner that is not properly reflected by bl...